Jaturakan Orapun, Buranakarl Chollada, Dissayabutra Thasinas, Chaiyabutr Narongsak, Kijtawornrat Anusak, Rungsipipat Anudep
Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Rd., Pathumwan, Bangkok 10330, Thailand.
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
J Vet Med Sci. 2017 Nov 17;79(11):1861-1869. doi: 10.1292/jvms.17-0340. Epub 2017 Sep 23.
Klotho protein is recognized as having a renoprotective effect and is used as a biomarker for kidney injury. We investigated the level of Klotho protein in hyperoxaluria-induced kidney injury and the effects of vitamin E (Vit E) and vitamin C (Vit C) supplementation. Hyperoxaluria was induced by feeding 2% (w/v) Hydroxy-L-proline (HLP) in the drinking water for 21 days. Rats were divided into 5 groups; control (Group 1, n=7), HLP treated rats that received nothing else (Group 2, n=7), Vit E (Group 3, n=6), Vit C (Group 4, n=6) and both Vit E and Vit C (Group 5, n=7). Vit E (200 mg/kg) was injected on days 1, 6, 11 and 16, while Vit C (500 mg/kg) was given intravenously on days 1 and 11. The Klotho protein levels and oxidative status were measured. The expression level of kidney Klotho protein expression was significantly reduced by HLP-treatment, while the mRNA expression was higher (P<0.05), the plasma and kidney malondialdehyde and kidney superoxide dismutase activities were increased, and the kidney reduced glutathione and urinary total antioxidant status were decreased (P<0.05). All of these changes were ameliorated by administration of Vit E, Vit C or especially the co-administration of both. In conclusion, HLP-induced hyperoxaluria reduced the kidney Klotho protein level, which could be restored by Vit E and/or Vit C.
klotho蛋白被认为具有肾脏保护作用，并被用作肾损伤的生物标志物。我们研究了高草酸尿症诱导的肾损伤中klotho蛋白的水平以及补充维生素E（Vit E）和维生素C（Vit C）的效果。通过在饮用水中喂食2%（w/v）的羟基-L-脯氨酸（HLP）21天来诱导高草酸尿症。将大鼠分为5组；对照组（第1组，n=7），仅接受HLP治疗的大鼠（第2组，n=7），Vit E组（第3组，n=6），Vit C组（第4组，n=6）以及Vit E和Vit C联合组（第5组，n=7）。在第1、6、11和16天注射Vit E（200mg/kg），而在第1和11天静脉注射Vit C（500mg/kg）。测量klotho蛋白水平和氧化状态。HLP治疗显著降低了肾脏klotho蛋白的表达水平，而mRNA表达较高（P<0.05），血浆和肾脏丙二醛以及肾脏超氧化物歧化酶活性增加，肾脏还原型谷胱甘肽和尿总抗氧化状态降低（P<0.05）。给予Vit E、Vit C或尤其是两者联合使用可改善所有这些变化。总之，HLP诱导的高草酸尿症降低了肾脏klotho蛋白水平，而Vit E和/或Vit C可以使其恢复。
