Hernández-Salmerón Julie E, Moreno-Hagelsieb Gabriel, Santoyo Gustavo
Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada.
J Genomics. 2017 Aug 24;5:91-98. doi: 10.7150/jgen.21588. eCollection 2017.
UM270 is a rhizosphere-colonizing bacterium that produces multiple diffusible and volatile compounds involved in plant growth-promoting activities. Strain UM270 exhibits excellent biocontrol capacities against diverse fungal pathogens In a previous study, the general UM270 genome characteristics were published. Here, we report a deeper analysis of its gene content and compare it to other strains to unveil the genetic elements that might explain UM270's great colonizing and plant growth-promoting capabilities. Our analyses found high variation in genome size and gene content among the eight genomes analyzed (strains UM270, Pf0-1, A506, F113, SBW25, PICF-7, UK4 and UW4). A core genome of 3,039 coding DNA sequences (CDSs) was determined, with 599 CDSs present only in the UM270 genome. From these unique UM270 genes, a set of 192 CDSs was found to be involved in signaling, rhizosphere colonization and competence, highlighted as important traits to achieve an effective biocontrol and plant growth promotion.
UM270是一种定殖于根际的细菌，它能产生多种参与促进植物生长活动的可扩散和挥发性化合物。UM270菌株对多种真菌病原体具有出色的生物防治能力。在之前的一项研究中，公布了UM270的一般基因组特征。在此，我们报告对其基因内容进行更深入的分析，并将其与其他菌株进行比较，以揭示可能解释UM270强大的定殖和促进植物生长能力的遗传元件。我们的分析发现，在所分析的8个基因组（UM270、Pf0-1、A506、F113、SBW25、PICF-7、UK4和UW4菌株）中，基因组大小和基因内容存在很大差异。确定了一个由3039个编码DNA序列（CDS）组成的核心基因组，其中599个CDS仅存在于UM270基因组中。从这些UM270特有的基因中，发现一组192个CDS参与信号传导、根际定殖和感受态，这些被视为实现有效生物防治和促进植物生长的重要性状。
