Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland.
Int J Mol Sci. 2020 Nov 19;21(22):8740. doi: 10.3390/ijms21228740.
Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of BRZ63, a new endophyte of oilseed rape ( L.) against W70, K, K2291, and . In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.
内生细菌在作为生物防治剂方面具有巨大的潜力。我们的研究旨在研究 BRZ63（一种新型油菜内生菌）对 W70、K、K2291 和 的生物防治活性。此外，还评估了对生物防治至关重要的特征、植物生长促进和定殖能力，并将其与基因组序列相关联。体外试验表明，BRZ63 显著抑制了所有测试病原体的菌丝生长，并刺激了用真菌病原体处理的油菜幼苗的萌发和生长。BRZ63 菌株可以通过产生生物表面活性剂、铁载体、吲哚-3-乙酸（IAA）、1-氨基环丙烷-1-羧酸（ACC）脱氨酶和氨以及溶解磷酸盐来使植物受益。此外，胞外多糖产生、自动聚集和生物膜形成的能力进一步强调了其对植物定殖和生物防治的潜力。通过观察 EGFP 表达细胞定殖于 Col-0 的根表面和表皮细胞，证实了 BRZ63 菌株的有效定殖特性。基因组挖掘鉴定了许多与生物防治过程相关的基因，如转运蛋白、铁载体和其他次生代谢物。所有分析都表明，BRZ63 菌株是一种优秀的内生候选生物防治各种植物病原体和促进植物生长的菌株。
