Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States.
Elife. 2017 Sep 25;6:e29839. doi: 10.7554/eLife.29839.
Studies of neuronal network emergence during sensory processing and motor control are greatly facilitated by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging, we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.
在感官处理和运动控制过程中，神经元网络的出现研究极大地受益于能够以单细胞分辨率同时记录大量神经元膜电位动力学的技术。为了实现具有检测小突触电位和动作电位能力的全脑记录，我们开发了一种基于双面显微镜的电压敏感染料 (VSD) 成像技术，该技术能够同时对神经系统的两面进行成像。我们将该系统应用于医用水蛭的节段性神经节。双面 VSD 成像使我们能够同时记录几乎所有可识别神经元的膜电位事件。利用从双面 VSD 成像获得的数据，我们分析了感觉处理和行为产生过程中的神经元动力学，并构建了功能图谱以鉴定对这些过程有贡献的神经元。
