Li Yong, Fang Wei, Xue Han, Yang Xu-Qi, Xie Shou-Jiang, Wang Lai-Fa
The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Forestry Bureau of Taishan, Taishan 529200, PR China.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4424-4428. doi: 10.1099/ijsem.0.002307. Epub 2017 Sep 25.
A Gram-stain-negative, facultatively anaerobic, motile, bacterial strain, 34C10-3-10, was isolated from symptomatic bark tissue of a Populus ×euramericana canker. The isolate could grow between 10 and 37 °C, at pH 5 to 11, and in 0-3 % (w/v) NaCl. The strain was oxidase and catalase positive. The ubiquinone of strain 34C10-3-10 was Q-8. The polar lipid profile of strain 34C10-3-10 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipid, phosphatidylmonomethylethanolamine, phosphatidylserine; the major fatty acids were C18 : 1ω7c, C14 : 0 and C16 : 1ω7c/C16 : 1ω6c. The average nucleotide identity (ANI) values between strain 34C10-3-10 and the type strains of reference species Wohlfahrtiimonas chitiniclastica S5 and Wohlfahrtiimonas larvae KBL006 were lower than the proposed species boundary cut-off for ANI. The DNA G+C content was 37.1 mol%. Based on phenotypic and genotypic characteristics, strain 34C10-3-10 represents a novel species of genus Wohlfahrtiimonas; the name Wohlfahrtiimonas populi sp. nov. is proposed. The type strain is 34C10-3-10 (=CFCC 12747=KCTC 52796).
从美洲黑杨溃疡病有症状的树皮组织中分离出一株革兰氏阴性、兼性厌氧、具运动性的细菌菌株34C10-3-10。该分离株能在10至37°C、pH值5至11以及0-3%（w/v）NaCl条件下生长。该菌株氧化酶和过氧化氢酶呈阳性。菌株34C10-3-10的泛醌为Q-8。菌株34C10-3-10的极性脂质谱包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂、磷脂酰单甲基乙醇胺、磷脂酰丝氨酸；主要脂肪酸为C18 : 1ω7c、C14 : 0和C16 : 1ω7c/C16 : 1ω6c。菌株34C10-3-10与参考物种几丁质分解沃氏菌S5和幼虫沃氏菌KBL006的模式菌株之间的平均核苷酸同一性（ANI）值低于提议的ANI物种界限截止值。DNA G+C含量为37.1 mol%。基于表型和基因型特征，菌株34C10-3-10代表沃氏菌属的一个新物种；提议命名为杨树沃氏菌（Wohlfahrtiimonas populi sp. nov.）。模式菌株为34C10-3-10（=CFCC 12747=KCTC 52796）。
