施用尿素促进满江红中的氨基酸代谢和膜脂过氧化。

Urea application promotes amino acid metabolism and membrane lipid peroxidation in Azolla.

作者信息

Chen Jiana, Huang Min, Cao Fangbo, Pardha-Saradhi P, Zou Yingbin

机构信息

Southern Regional Collaborative Innovation Center for Grain and Oil Crops (CICGO), Hunan Agricultural University, Changsha, China.

Department of Environmental Studies, University of Delhi, Delhi, India.

出版信息

PLoS One. 2017 Sep 25;12(9):e0185230. doi: 10.1371/journal.pone.0185230. eCollection 2017.

DOI:10.1371/journal.pone.0185230

PMID:28945775

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5612470/

Abstract

A pot experiment was conducted to evaluate the effect of urea on nitrogen metabolism and membrane lipid peroxidation in Azolla pinnata. Compared to controls, the application of urea to A. pinnata resulted in a 44% decrease in nitrogenase activity, no significant change in glutamine synthetase activity, 660% higher glutamic-pyruvic transaminase, 39% increase in free amino acid levels, 22% increase in malondialdehyde levels, 21% increase in Na+/K+- levels, 16% increase in Ca2+/Mg2+-ATPase levels, and 11% decrease in superoxide dismutase activity. In terms of H2O2 detoxifying enzymes, peroxidase activity did not change and catalase activity increased by 64% in urea-treated A. pinnata. These findings suggest that urea application promotes amino acid metabolism and membrane lipid peroxidation in A. pinnata.

摘要

进行了一项盆栽试验，以评估尿素对满江红氮代谢和膜脂过氧化的影响。与对照相比，向满江红施用尿素导致固氮酶活性降低44%，谷氨酰胺合成酶活性无显著变化，谷丙转氨酶升高660%，游离氨基酸水平增加39%，丙二醛水平增加22%，Na⁺/K⁺水平增加21%，Ca²⁺/Mg²⁺-ATP酶水平增加16%，超氧化物歧化酶活性降低11%。就H₂O₂解毒酶而言，在施用尿素的满江红中，过氧化物酶活性没有变化，而过氧化氢酶活性增加了64%。这些发现表明，施用尿素促进了满江红的氨基酸代谢和膜脂过氧化。