Bliss T V, Errington M L, Laroche S, Lynch M A
Division of Neurophysiology and Neuropharmacology, National Institute for Medical Research, Mill Hill, London, U.K.
Neurosci Lett. 1987 Dec 16;83(1-2):107-12. doi: 10.1016/0304-3940(87)90224-2.
Long-term potentiation (LTP) was induced by unilateral tetanic stimulation of the perforant path in the dentate gyrus of rats with implanted electrodes. Evoked potentials were monitored for the subsequent 3 days in one group, and for 23 days in another. The dentate gyrus was removed bilaterally and slices prepared and stored in 10% DMSO/Krebs for subsequent analysis of K+-stimulated, Ca2+-dependent release of [3H]glutamate. In the 3-day group, in which the mean increase in the population EPSP was 35% at the time of sacrifice, release from the tetanized side was significantly greater than from the unstimulated side. In the 23-day group, both the increase in the EPSP and the increase in release from the tetanized side had declined to statistically insignificant levels. These results extend to a period of several days the previously observed association between LTP and increased K+-stimulated, Ca2+-dependent release of transmitter.
通过对植入电极的大鼠齿状回的穿通通路进行单侧强直刺激来诱导长时程增强（LTP）。一组在随后3天监测诱发电位，另一组在23天监测。双侧切除齿状回，制备切片并保存在10%二甲基亚砜/ Krebs液中，用于随后分析钾离子刺激、钙离子依赖的[3H]谷氨酸释放。在3天组中，处死时群体兴奋性突触后电位（EPSP）的平均增加为35%，强直刺激侧的释放显著大于未刺激侧。在23天组中，EPSP的增加以及强直刺激侧释放的增加均降至无统计学意义的水平。这些结果将先前观察到的LTP与递质释放增加之间的关联扩展到了数天的时间段。
