State Key Laboratory of Microbial Technology and National Glycoengineering Research Center, Shandong University, Jinan 250100, China; School of Life Science, Qufu Normal University, Qufu 273165, China.
State Key Laboratory of Microbial Technology and National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
Bioresour Technol. 2017 Dec;245(Pt A):984-992. doi: 10.1016/j.biortech.2017.09.042. Epub 2017 Sep 8.
Spent ammonium sulphite liquor (SASL) is the main effluent from the ammonium sulphite pulping process, and contains amounts of lignocellulosic oligomers, monosaccharides and ammonium salts. The effect of continuous SASL-feeding on cellulase production by Penicillium oxalicum was studied. With a rate-varying feeding strategy, the maximal filter paper enzyme (FPase) activity reached 17.66U/mL at 144h, and the specific FPase activity increased from 1.74U/mg (without SASL) to 2.40U/mg. Accordingly, the glucan hydrolysis conversion of delignified corn cob residue by the enzymes from continuous SASL-feeding fermentation was significantly higher than that without SASL at equal protein loadings. Comparative proteomic analysis demonstrated that the proteins involved in lignocellulose degradation were specifically up-regulated in the crude cellulase with SASL-feeding. The obtained crude enzyme was efficient in the hydrolysis of pulping products, with a glucan conversion of 81.87% achieved after 72h saccharification of ammonium sulphite pulp.
废亚硫酸铵液（SASL）是亚硫酸盐制浆过程的主要废水，含有木质纤维素低聚物、单糖和铵盐。研究了连续 SASL 进料对草酸青霉产纤维素酶的影响。采用变速进料策略，在 144h 时达到了 17.66U/mL 的最大滤纸酶（FPase）活性，比没有 SASL 时的 1.74U/mg（没有 SASL）提高了 2.40U/mg。相应地，在相同蛋白负载下，来自连续 SASL 进料发酵的酶对木质素脱除玉米芯残渣的葡聚糖水解转化率明显高于没有 SASL 的情况。比较蛋白质组学分析表明，在具有 SASL 进料的粗纤维素酶中，参与木质纤维素降解的蛋白质被特异性地上调。获得的粗酶在制浆产物的水解中效率很高，经过 72h 的亚硫酸盐浆糖化后，葡萄糖转化率达到 81.87%。
