Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources and Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
School of Biology and Environment, Nanjing Polytechnic Institute, 625 Geguan Road, Nanjing 210048, Jiangsu, China; School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
Bioresour Technol. 2019 Sep;287:121417. doi: 10.1016/j.biortech.2019.121417. Epub 2019 May 4.
Herbel-tolerant strains exhibit considerable environmental and commercial values not only due to their harmless treatment of herbal-extraction residues (HERs) but also because of their use in preparing high-quality cellulase cocktails. In this study, three typical HERs were evaluated for enzymatic in situ saccharification performance. A HERs-tolerance fungus, identified as Penicillium oxalicum G2, can grow in 1.5% (w/v) Radix isatidis residues (RIR), thereby exhibiting the highest FPase (2.2 U/mL), carboxymethyl cellulase (13.3 U/mL), and β-glucosidase (4.6 U/mL) activities. The most effective production of cellulase cocktail was achieved via orthogonal experiment in a system with pH 6.0, 30 °C, and 96 h. Cellulase cocktail from P. oxalicum G2 can directly saccharify the extraction RIR, thereby achieving a maximum reducing sugar yield of 7.2 mg/mL, which is 1.7-fold higher than those of commercial cellulases. Results illustrate the potential of P. oxalicum G2 for enzymatic in situ saccharification.
具有耐草甘膦特性的菌株不仅由于其对草药提取残留物（HERs）的无害处理，而且由于其在制备高质量纤维素酶混合物中的用途，具有相当大的环境和商业价值。在这项研究中，评估了三种典型的 HERs 的酶原位糖化性能。一种鉴定为草酸青霉 G2 的耐 HERs 真菌可以在 1.5%（w/v）板蓝根残留物（RIR）中生长，从而表现出最高的 FPase（2.2 U/mL）、羧甲基纤维素酶（13.3 U/mL）和β-葡萄糖苷酶（4.6 U/mL）活性。通过在 pH 值为 6.0、30°C 和 96 h 的系统中的正交实验，实现了纤维素酶混合物的最有效生产。来自 P. oxalicum G2 的纤维素酶混合物可以直接糖化提取的 RIR，从而实现最大还原糖产量为 7.2 mg/mL，比商业纤维素酶高 1.7 倍。结果表明，P. oxalicum G2 具有酶原位糖化的潜力。
