乳腺和肺肿块针吸活检中中期因子和多效蛋白浓度。
Midkine and pleiotrophin concentrations in needle biopsies of breast and lung masses.
机构信息
Department of Pediatrics, Walter Reed National Military Medical Center, Bethesda, MD, USA.
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
出版信息
Cancer Biomark. 2017 Sep 7;20(3):299-307. doi: 10.3233/CBM-170145.
BACKGROUND/OBJECTIVE: Midkine (MDK) and pleiotrophin (PTN) are two closely related heparin-binding growth factors which are overexpressed in a wide variety of human cancers. We hypothesized that the concentrations of these factors in washout of biopsy needles would be higher in breast and lung cancer than in benign lesions.
METHODS
Seventy subjects underwent pre-operative core needle biopsies of 78 breast masses (16 malignancies). In 11 subjects, fine needle aspiration was performed ex vivo on 7 non-small cell lung cancers and 11 normal lung specimens within surgically excised lung tissue. The biopsy needle was washed with buffer for immunoassay.
RESULTS
The MDK/DNA and the PTN/DNA ratio in most of the malignant breast masses were similar to the ratios in benign masses except one lobular carcinoma in situ (24-fold higher PTN/DNA ratio than the average benign mass). The MDK/DNA and PTN/DNA ratio were similar in most malignant and normal lung tissue except one squamous cell carcinoma (38-fold higher MDK/DNA ratio than the average of normal lung tissue).
CONCLUSIONS
Both MDK and PTN are readily measurable in washout of needle biopsy samples from breast and lung masses and levels are highly elevated only in a specific subset of these malignancies.
背景/目的：中期因子（MDK）和多效蛋白（PTN）是两种密切相关的肝素结合生长因子，在多种人类癌症中过度表达。我们假设这些因子在活检针冲洗液中的浓度在乳腺癌和肺癌中会高于良性病变。
方法
70 名受试者接受了 78 个乳腺肿块（16 个恶性肿瘤）的术前核心针活检。在 11 名受试者中，在手术切除的肺组织中对 7 个非小细胞肺癌和 11 个正常肺标本进行了体外细针抽吸。用缓冲液冲洗活检针以进行免疫测定。
结果
大多数恶性乳腺肿块的 MDK/DNA 和 PTN/DNA 比值与良性肿块相似，除了一个原位小叶癌（PTN/DNA 比值比平均良性肿块高 24 倍）。大多数恶性和正常肺组织的 MDK/DNA 和 PTN/DNA 比值相似，除了一个鳞状细胞癌（MDK/DNA 比值比正常肺组织的平均值高 38 倍）。
结论
MDK 和 PTN 均可在乳腺和肺肿块的针吸活检样本冲洗液中进行定量检测，并且仅在这些恶性肿瘤的特定亚组中显著升高。
Zotero 插件