Travers A A, Klug A
Medical Research Council Laboratory of Molecular Biology, Cambridge, U.K.
Philos Trans R Soc Lond B Biol Sci. 1987 Dec 15;317(1187):537-61. doi: 10.1098/rstb.1987.0080.
The DNA of a nucleosome core particle is wrapped tightly around a histone octamer with approximately 80 base pairs per superhelical turn. Studies of both naturally occurring and reconstituted systems have shown that DNA sequences very often adopt well-defined locations with respect to the octamer. Recent work in this laboratory has provided a structural explanation for this sequence-dependent positioning in terms of the differential flexibility of different sequences and of departures from smooth bending. The 'rules' that are emerging for DNA bendability and, from the results of other workers, on intrinsically bent DNA, are likely to be useful in considering looping and bending of DNA in other processes in which it is thought to be wrapped around a protein core.
核小体核心颗粒的DNA紧密缠绕在组蛋白八聚体周围，每个超螺旋圈约有80个碱基对。对天然存在的和重组系统的研究表明，DNA序列相对于八聚体常常采用明确的定位。本实验室最近的工作从不同序列的差异柔韧性以及偏离平滑弯曲的角度，为这种序列依赖性定位提供了结构解释。关于DNA可弯曲性以及从其他研究者对固有弯曲DNA的研究结果中得出的“规则”，在考虑DNA在其他被认为围绕蛋白质核心缠绕的过程中的环化和弯曲时可能会很有用。
