Borchel Andreas, Verleih Marieke, Rebl Alexander, Goldammer Tom
Leibniz Institute for Farm Animal Biology (FBN), Institute of Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
J Genet. 2017 Sep;96(4):701-706. doi: 10.1007/s12041-017-0811-x.
A rapid decline in temperature poses a major challenge for poikilothermic fish, as their entire metabolism depends on ambient temperature. The gene expression of rainbow trout Oncorhynchus mykiss having undergone such a cold shock (0◦C) was compared to a control (5◦C) in a microarray and quantitative real-time PCR based study. The tissues of gill, kidney and liver were examined. The most differently expressed genes were found in liver, many of them contributing to the network 'cellular compromise, cellular growth and proliferation'.However, the number of genes found to be regulated at 0◦Cwas surprisingly low. Instead of classical genes involved in temperature shock, the three genes encoding fibroblast growth factor 1 (fgf1), growth arrest and DNA-damageinducible, alpha (gadd45a) and sclerostin domain-containing protein 1 (sostdc1) were upregulated in the liver upon cold shock in two different rainbow trout strains, suggesting that these genes may be considered as general biomarkers for cold shock in rainbow trout.
温度的快速下降给变温鱼类带来了重大挑战，因为它们的整个新陈代谢都依赖于环境温度。在一项基于微阵列和定量实时PCR的研究中，将经历了这种冷休克（0°C）的虹鳟鱼（Oncorhynchus mykiss）的基因表达与对照组（5°C）进行了比较。对鳃、肾脏和肝脏组织进行了检查。在肝脏中发现了表达差异最大的基因，其中许多基因参与了“细胞损伤、细胞生长和增殖”网络。然而，发现在0°C时受调控的基因数量出奇地少。在两种不同的虹鳟鱼品系中，冷休克后肝脏中编码成纤维细胞生长因子1（fgf1）、生长停滞和DNA损伤诱导蛋白α（gadd45a）以及含硬化蛋白结构域蛋白1（sostdc1）的三个基因上调，而不是涉及温度休克的经典基因，这表明这些基因可能被视为虹鳟鱼冷休克的通用生物标志物。
