Mutoh N, Hayashi Y
Department of Genetics, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Biochem Biophys Res Commun. 1988 Feb 29;151(1):32-9. doi: 10.1016/0006-291x(88)90555-4.
Schizosaccharomyces pombe synthesize small cadmium-binding peptides cadystin, structure of which is (gamma-Glu-Cys)n-Gly, in response to cadmium. Mutants unable to synthesize cadystin were found in the mutants hypersensitive to cadmium. Some of them lack activity of either gamma-glutamylcysteine synthetase (EC 6.3.2.2) or glutathione synthetase (EC 6.3.2.3), enzyme involved in glutathione biosynthesis. Some mutants have the same activity levels of these enzymes as wild type has. These results indicate that some steps of cadystin biosynthesis are catalyzed by the enzymes catalyzing glutathione biosynthesis.
粟酒裂殖酵母在镉胁迫下会合成小镉结合肽镉硫蛋白，其结构为（γ-谷氨酰-半胱氨酸）n-甘氨酸。在对镉超敏感的突变体中发现了无法合成镉硫蛋白的突变体。其中一些突变体缺乏γ-谷氨酰半胱氨酸合成酶（EC 6.3.2.2）或谷胱甘肽合成酶（EC 6.3.2.3）的活性，这两种酶参与谷胱甘肽的生物合成。一些突变体的这些酶活性水平与野生型相同。这些结果表明，镉硫蛋白生物合成的某些步骤是由催化谷胱甘肽生物合成的酶催化的。
