Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland.
Department of Medical Rehabilitation and Clinical Physiotherapy, Pomeranian Medical University, Szczecin, Poland.
Histol Histopathol. 2018 Apr;33(4):389-397. doi: 10.14670/HH-11-934. Epub 2017 Sep 26.
Apoptosis and proliferation of prostate cells are associated with both physiological increase and hyperplasia of the prostate. The aim of this study was to determine the contribution of metabolic syndrome to the processes of apoptosis and proliferation in gland epithelial cells and prostatic stromal cells in men with BPH.
The study involved 151 men, aged 52-89 years, receiving pharmacological treatment for BPH. The men were divided into two groups: those with and those without metabolic syndrome. The serum levels of the parameters were determined. Reactions for the identification of apoptosis (TUNEL) and proliferation (PCNA) in cells were also performed.
The relationships between the number of TUNEL(+) and PCNA(+) cells and metabolic syndrome were not observed. It was found that the total number of TUNEL(+) cells in the prostate stroma correlated negatively with the levels of high-density lipoprotein and insulin-like growth factor-1. The analysis of the correlations in BPH patients with and without metabolic syndrome demonstrated that the only parameter correlating with the number of PCNA(+) cells in the prostate stroma was insulin resistance.
Metabolic syndrome in patients with BPH had no impact on the number of TUNEL(+) and PCNA(+) cells in the prostate gland. However, the disturbed levels of metabolic parameters, and deviations of anthropometric parameters from normal may influence the number of apoptotic and proliferating cells.
前列腺细胞的凋亡和增殖与前列腺的生理增生和增生有关。本研究的目的是确定代谢综合征对 BPH 患者前列腺上皮细胞和基质细胞凋亡和增殖过程的贡献。
本研究涉及 151 名年龄在 52-89 岁之间的男性，他们正在接受 BPH 的药物治疗。这些男性被分为两组：有代谢综合征的组和没有代谢综合征的组。测定了血清参数水平。还进行了细胞凋亡（TUNEL）和增殖（PCNA）鉴定的反应。
未观察到 TUNEL(+)和 PCNA(+)细胞数量与代谢综合征之间的关系。结果发现，前列腺基质中总 TUNEL(+)细胞的数量与高密度脂蛋白和胰岛素样生长因子-1的水平呈负相关。在有和没有代谢综合征的 BPH 患者中进行的相关性分析表明，与前列腺基质中 PCNA(+)细胞数量相关的唯一参数是胰岛素抵抗。
BPH 患者的代谢综合征对前列腺中 TUNEL(+)和 PCNA(+)细胞的数量没有影响。然而，代谢参数水平的紊乱以及人体测量参数偏离正常值可能会影响凋亡和增殖细胞的数量。
