Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
Nanoscale. 2017 Oct 12;9(39):14897-14906. doi: 10.1039/c7nr03891g.
The increase of erythrocyte aggregation by high fibrinogen levels may be an indicator of cardiovascular risk. γ' fibrinogen variant has been considered as a possible player in enhancing aggregation. Here, we assessed, at the single-cell level, the influence of fibrinogen on erythrocyte aggregation in essential arterial hypertension. We also aimed at understanding how γ' fibrinogen is altered in this disease. Using atomic force microscopy (AFM), we show that the work and force necessary for erythrocyte-erythrocyte detachment is higher for patients than for healthy donors, with these parameters further increasing in both groups when higher fibrinogen concentrations are present. This can be associated with changes in blood flow, due to transient bridging of two erythrocytes by fibrinogen, representing an important cardiovascular risk factor. γ' fibrinogen can influence the increased risk in essential arterial hypertension, as we demonstrate that its levels are significantly increased in these patients' blood. Nevertheless, this cannot be the only cause for the changes observed in the AFM data.
高纤维蛋白原水平可导致红细胞聚集增加，这可能是心血管风险的一个指标。γ'纤维蛋白原变体被认为是增强聚集的一个可能因素。在这里，我们在单细胞水平上评估了纤维蛋白原对原发性高血压患者红细胞聚集的影响。我们还旨在了解γ'纤维蛋白原在这种疾病中是如何改变的。使用原子力显微镜（AFM），我们发现与健康供体相比，患者的红细胞-红细胞分离所需的功和力更高，当存在更高浓度的纤维蛋白原时，这两个参数在两个组中进一步增加。这可能与血流变化有关，因为纤维蛋白原暂时桥接了两个红细胞，这是一个重要的心血管危险因素。γ'纤维蛋白原可能会影响原发性高血压的高风险，正如我们所证明的，它在这些患者的血液中的水平显著升高。然而，这不可能是 AFM 数据中观察到的变化的唯一原因。
