Silva Laura A, Marques Lis S, Zhang Tiantian, Rodrigues Rômulo B, Eloy Lidiane Raquel, Streit Danilo P
Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91540-000, Brazil.
Department of Animal Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91540-000, Brazil.
Theriogenology. 2018 Jan 1;105:115-119. doi: 10.1016/j.theriogenology.2017.09.021. Epub 2017 Sep 21.
This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.
本研究旨在评估鲤鱼垂体提取物（CPE）、促卵泡激素（FSH）和促黄体生成素（LH）对斑马鱼卵母细胞成熟的影响，以及这些成熟卵母细胞受精并发育至孵化的能力。III期卵泡在八种处理中成熟：五种浓度的CPE（16、32、48、64和80μg/mL）、一种FSH（0.5μg/mL）、一种LH（0.5μg/mL）或一种FSH（0.5μg/mL）与LH（0.5μg/mL）的组合。CPE处理的成熟率分别为12.8%（16μg/mL）、24.8%（32μg/mL）、27.0%（48μg/mL）、22.7%（64μg/mL）和9.6%（80μg/mL）；FSH处理为15.7%（0.5μg/mL），LH处理为31.8%（0.5μg/mL），FSH（0.5μg/mL）与LH（0.5μg/mL）组合处理为50.4%。所有处理均进行了体外受精；然而，只有FSH和LH组合处理产生了受精卵。使用FSH与LH组合成熟后，分裂率为33.3%，活幼虫孵化率为20.0%。这些结果表明，FSH与LH组合在斑马鱼卵母细胞的体外成熟中是有效的。
