Jiao Jiao, Gai Qing-Yan, Niu Li-Li, Wang Xi-Qing, Guo Na, Zang Yu-Ping, Fu Yu-Jie
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University , Harbin 150040, People's Republic of China.
Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University , Beijing 100083, People's Republic of China.
J Agric Food Chem. 2017 Oct 18;65(41):9078-9086. doi: 10.1021/acs.jafc.7b03148. Epub 2017 Oct 5.
A cocultivation system of Astragalus membranaceus hairy root cultures (AMHRCs) and immobilized food-grade fungi was established for the enhanced production of calycosin (CA) and formononetin (FO). The highest accumulations of CA (730.88 ± 63.72 μg/g DW) and FO (1119.42 ± 95.85 μg/g DW) were achieved in 34 day-old AMHRCs cocultured with immobilized A. niger (IAN) for 54 h, which were 7.72- and 18.78-fold higher than CA and FO in nontreated control, respectively. IAN deglycosylation could promote the formation of CA and FO by conversion of their glycoside precursors. IAN elicitation could intensify the generation of endogenous signal molecules involved in plant defense response, which contributed to the significantly up-regulated expression of genes in CA and FO biosynthetic pathway. Overall, the coupled culture of IAN and AMHRCs offered a promising and effective in vitro approach to enhance the production of two health-promoting isoflavone aglycones for possible nutraceutical and pharmaceutical uses.
建立了黄芪毛状根培养物（AMHRCs）与固定化食品级真菌的共培养系统，以提高毛蕊异黄酮（CA）和芒柄花素（FO）的产量。在与固定化黑曲霉（IAN）共培养54小时的34日龄AMHRCs中，CA（730.88±63.72μg/g干重）和FO（1119.42±95.85μg/g干重）的积累量最高，分别比未处理对照中的CA和FO高7.72倍和18.78倍。IAN去糖基化可通过其糖苷前体的转化促进CA和FO的形成。IAN诱导可增强参与植物防御反应的内源性信号分子的产生，这有助于CA和FO生物合成途径中基因的显著上调表达。总体而言，IAN与AMHRCs的联合培养提供了一种有前景且有效的体外方法，以提高两种具有健康促进作用的异黄酮苷元的产量，用于可能的营养保健品和药物用途。
