高效液相色谱法测定膜荚黄芪毛状根培养物中的6种异黄酮类成分

[Determination of 6 isoflavonoids in the hairy root cultures of Astragalus membranaceus by HPLC].

作者信息

Zheng Z, Song C, Liu D, Hu Z

机构信息

Laboratory of Biotechnology of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine and Pharmacy, Shanghai 200032.

出版信息

Yao Xue Xue Bao. 1998;33(2):148-51.

PMID:11938951

Abstract

An accurate RP-HPLC method for the determination of 10-hydroxy-3, 9-dimethoxypeterocarpan, (3R) 8,2'-dihydroxy-7,4'-dimethoxyisoflavan, formonenetin (7-hydroxy-4'-methoxyisoflavone), 8, 3'-dihydroxy-7, 4'-dimethoxy-isoflavone, 2'-hydroxy-3', 4'-dimethoxyisoflavan-7-O-glucopyranoside and alycosin (7,3'-dihydroxy-4'-methoxyisoflavone) in the hairy root cultures of Astragalus membranaceus was developed. Among them, 10-hydroxy-3, 9-dimethoxypeterocarpan, (3R) 8,2'-dihydroxy-7,4'-dimethoxyisoflavan and 8,3'-dihydroxy-7,4'-dimethoxyisoflavone were new compounds in Astragalus membranaceus. The analytical column was Nucleosil C18. The mobile phases were methanol-water = 3:2 and 1:1 (v/v). The detection wavelengths were 254 nm and 280 nm. Calibration graphs were rectilinear between 2.5 micrograms and 12.5 micrograms. The recoveries were between 96.47% and 103.33%. The relative standard deviations (RSDs) of measurement precision test were 2.57%-6.52%. The flavonoid contents in the hairy root cultures were between 0.0005% and 0.0065% (dry weight).

摘要

建立了一种准确的反相高效液相色谱（RP-HPLC）法，用于测定膜荚黄芪毛状根培养物中的10-羟基-3,9-二甲氧基紫穗槐二氢黄酮、（3R）8,2'-二羟基-7,4'-二甲氧基异黄酮、芒柄花黄素（7-羟基-4'-甲氧基异黄酮）、8,3'-二羟基-7,4'-二甲氧基异黄酮、2'-羟基-3',4'-二甲氧基异黄酮-7-O-葡萄糖苷和阿福豆苷（7,3'-二羟基-4'-甲氧基异黄酮）。其中，10-羟基-3,9-二甲氧基紫穗槐二氢黄酮、（3R）8,2'-二羟基-7,4'-二甲氧基异黄酮和8,3'-二羟基-7,4'-二甲氧基异黄酮是膜荚黄芪中的新化合物。分析柱为Nucleosil C18。流动相为甲醇-水=3:2和1:1（v/v）。检测波长为254 nm和280 nm。校准曲线在2.5微克至12.5微克之间呈线性。回收率在96.47%至103.33%之间。测量精密度试验的相对标准偏差（RSD）为2.57%-6.52%。毛状根培养物中的黄酮类化合物含量在0.0005%至0.0065%（干重）之间。