Department of Oncology, Jinshan Hospital, Shanghai Medical College, Fudan University, 1508 Longhang Road, Shanghai, 201508, People's Republic of China.
Department of Radiology, Jinshan Hospital, Shanghai Medical College, Fudan University, 1508 Longhang Road, Shanghai, 201508, People's Republic of China.
J Ovarian Res. 2017 Sep 26;10(1):65. doi: 10.1186/s13048-017-0362-z.
To investigate dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) for assessing histopathological and molecular biological features in induced rat epithelial ovarian carcinomas (EOCs).
7,12-dimethylbenz[A]anthracene (DMBA) was applied to induce EOCs in situ in 46 SD rats. Conventional MRI and DCE-MRI were performed to evaluate the morphology and perfusion features of the tumors, including the time-signal intensity curve (TIC), volume transfer constant (K), rate constant (K), extravascular extracellular space volume ratio (V) and initial area under the curve (IAUC). DCE-MRI parameters were correlated with histological grade, microvascular density (MVD), vascular endothelial growth factor (VEGF) and fraction of Ki67-positive cells and the serum level of cancer antigen 125 (CA125).
Thirty-five of the 46 rats developed EOCs. DCE-MRI showed type III TIC more frequently than type II (29/35 vs. 6/35, p < 0.001) in EOCs. The two types of TIC of tumors had significant differences in the histological grade, MVD, expression of VEGF and Ki67, and the serum level of CA125 (all p < 0.01). K, K and IAUC values showed significant differences in different histological grades in overall and pairwise comparisons except for IAUC in grade 2 vs. grade 3 (all p < 0.01). There was no significant difference in V values among the three grade groups (p > 0.05). K, K and IAUC values were positively correlated with MVD, VEGF and Ki67 expression (all p < 0.01). V was not significantly correlated with MVD, VEGF expression, Ki67 expression and the CA125 level (all p > 0.05).
TIC types and perfusion parameters of DCE-MRI can reflect tumor grade, angiogenesis and cell proliferation to some extent, thereby helping treatment planning and predicting prognosis.
探讨动态对比增强磁共振成像（DCE-MRI）在评估诱导性大鼠卵巢上皮性癌（EOC）的组织病理学和分子生物学特征中的作用。
采用 7,12-二甲基苯并蒽（DMBA）原位诱导 46 只 SD 大鼠发生 EOC。对肿瘤的形态学和灌注特征进行常规 MRI 和 DCE-MRI 检查，包括时间信号强度曲线（TIC）、容积转移常数（K）、速率常数（K）、血管外细胞外容积比（V）和初始曲线下面积（IAUC）。DCE-MRI 参数与组织学分级、微血管密度（MVD）、血管内皮生长因子（VEGF）、Ki67 阳性细胞比例以及血清癌抗原 125（CA125）水平相关。
46 只大鼠中 35 只发展为 EOC。DCE-MRI 显示 EOC 中 TIC 类型 III 比类型 II 更常见（29/35 比 6/35，p<0.001）。两种 TIC 类型的肿瘤在组织学分级、MVD、VEGF 和 Ki67 的表达以及 CA125 血清水平方面存在显著差异（均 p<0.01）。除 2 级与 3 级之间的 IAUC 外（均 p<0.01），在总体和两两比较中，不同组织学分级的 K、K 和 IAUC 值存在显著差异。三组间 V 值无显著差异（p>0.05）。K、K 和 IAUC 值与 MVD、VEGF 表达和 Ki67 表达呈正相关（均 p<0.01）。V 与 MVD、VEGF 表达、Ki67 表达和 CA125 水平无显著相关性（均 p>0.05）。
DCE-MRI 的 TIC 类型和灌注参数在一定程度上可以反映肿瘤分级、血管生成和细胞增殖，有助于治疗计划和预测预后。
