Chen Juan, Qian Ting, Zhang Huanhuan, Wei Chunxiao, Meng Fanhua, Yin Huabin
Department of Radiology, The Fifth People of Shanghai, Fudan University, Shanghai, China.
Department of Radiology, The Fifth People of Shanghai, Fudan University, Shanghai, China.
Magn Reson Imaging. 2016 Feb;34(2):177-82. doi: 10.1016/j.mri.2015.10.013. Epub 2015 Oct 27.
To evaluate the correlation between parameters of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) and microvessel density (MVD) measurements in rabbit VX2 liver tumor models after percutaneous ethanol injection (PEI) and to observe influence of PEI on angiogenesis in a rabbit VX2 liver tumor model with dynamic contrast enhanced magnetic resonance imaging (DCE-MRI).
Forty five New Zealand white rabbits were used in this study. VX2 tumor tissue blocks were implanted in the left lobe of liver by percutaneous puncture under CT guidance. 2 weeks later, all rabbits underwent conventional MRI (T1WI, T2WI) to determine the successful models. Then those successful implanted VX2 liver tumor models in the study were randomly divided into the control group and the experimental group, the former did not have processing, and the latter underwent PEI under CT guidance. MRI (T1WI, T2WI and DCE-MRI) was performed 1 week later again, the parameters of DCE-MRI (Ktrans, Kep, Ve and iAUC60) of viable tumor portions were observed. Then all the liver samples were processed for hematoxylin and eosin (H&E) staining and immunohistochemical staining for CD31 to determine MVD. At last, data (including DCE-MRI perfusion parameters and MVD) were compared between experimental and control groups, correlation of DCE-MRI perfusion parameters and MVD was evaluated.
Twenty six VX2 liver tumor models underwent all examinations (thirteen models for each group) 1 week later after PEI. For the experimental group, the parameters Ktrans (r=0.6382, P=0.0189) and iAUC60 (r=0.6591, P=0.0143) in viable tumor portions were positively moderately correlated with MVD, whereas the parameters Kep (r=0.4656, P=0.1088) and Ve (r=0.2918, P=0.3333) were not correlated with MVD. For the control group, the parameters Ktrans (r=0.6385, P=0.0188) and iAUC60 (r=0.6391, P=0.0187) in viable tumor portions were also positively moderately correlated with MVD, while the parameters Kep (r=0.5518, P=0.0506) and Ve (r=-0.0824, P=0.789) were not correlated with MVD. Ktrans, Kep, Ve, iAUC60 and MVD of residual viable tumors in the experimental group 1 week later after PEI were similar to the viable tumors of the control group (P>0.05).
DCE-MRI could be used to evaluate the efficiency of VX2 liver tumor after PEI. The quantitative parameter Ktrans and semi-quantitative parameter iAUC60 of DCE-MRI are correlated with MVD, which can assess tumor angiogenesis noninvasively of VX2 liver tumor model, and ethanol has no significant impact on angiogenesis of viable tumor 1week later after PEI.
评估经皮乙醇注射（PEI）后兔VX2肝肿瘤模型中动态对比增强磁共振成像（DCE-MRI）参数与微血管密度（MVD）测量值之间的相关性，并通过动态对比增强磁共振成像（DCE-MRI）观察PEI对兔VX2肝肿瘤模型血管生成的影响。
本研究使用45只新西兰白兔。在CT引导下经皮穿刺将VX2肿瘤组织块植入肝脏左叶。2周后，所有兔子均接受常规MRI（T1WI、T2WI）检查以确定模型是否成功建立。然后将研究中成功植入VX2肝肿瘤模型的兔子随机分为对照组和实验组，前者不进行处理，后者在CT引导下接受PEI。1周后再次进行MRI（T1WI、T2WI和DCE-MRI）检查，观察存活肿瘤部分的DCE-MRI参数（Ktrans、Kep、Ve和iAUC60）。然后对所有肝脏样本进行苏木精-伊红（H&E）染色和CD31免疫组织化学染色以确定MVD。最后，比较实验组和对照组的数据（包括DCE-MRI灌注参数和MVD），评估DCE-MRI灌注参数与MVD的相关性。
26个VX2肝肿瘤模型在PEI后1周接受了所有检查（每组13个模型）。对于实验组，存活肿瘤部分的参数Ktrans（r = 0.6382，P = 0.0189）和iAUC60（r = 0.6591，P = 0.0143）与MVD呈中度正相关，而参数Kep（r = 0.4656，P = 0.1088）和Ve（r = 0.2918，P = 0.3333）与MVD无相关性。对于对照组，存活肿瘤部分的参数Ktrans（r = 0.6385，P = 0.0188）和iAUC60（r = 0.6391，P = 0.0187）也与MVD呈中度正相关，而参数Kep（r = 0.5518，P = 0.0506）和Ve（r = -0.0824，P = 0.789）与MVD无相关性。PEI后1周实验组残余存活肿瘤的Ktrans、Kep、Ve、iAUC60和MVD与对照组的存活肿瘤相似（P>0.05）。
DCE-MRI可用于评估PEI后VX2肝肿瘤的疗效。DCE-MRI的定量参数Ktrans和半定量参数iAUC60与MVD相关，可无创评估VX2肝肿瘤模型的肿瘤血管生成，且乙醇在PEI后1周对存活肿瘤的血管生成无显著影响。
