Pittmann Timo, Steinmetz Heidrun
TBF + Partner AG, Herrenberger Strasse 14, 71032 Boeblingen, Germany.
Department of Resource Efficient Wastewater Technology, University of Kaiserslautern, Paul-Ehrlich-Str. 14, 67663 Kaiserslautern, Germany.
Bioengineering (Basel). 2017 Jun 6;4(2):54. doi: 10.3390/bioengineering4020054.
This work describes the production of polyhydroxyalkanoates (PHA) as a side stream process on a municipal waste water treatment plant (WWTP) and a subsequent analysis of the production potential in Germany and the European Union (EU). Therefore, tests with different types of sludge from a WWTP were investigated regarding their volatile fatty acids (VFA) production-potential. Afterwards, primary sludge was used as substrate to test a series of operating conditions (temperature, pH, retention time (RT) and withdrawal (WD)) in order to find suitable settings for a high and stable VFA production. In a second step, various tests regarding a high PHA production and stable PHA composition to determine the influence of substrate concentration, temperature, pH and cycle time of an installed feast/famine-regime were conducted. Experiments with a semi-continuous reactor operation showed that a short RT of 4 days and a small WD of 25% at pH = 6 and around 30 °C is preferable for a high VFA production rate (PR) of 1913 mgVFA/(L×d) and a stable VFA composition. A high PHA production up to 28.4% of cell dry weight (CDW) was reached at lower substrate concentration, 20 °C, neutral pH-value and a 24 h cycle time. A final step a potential analysis, based on the results and detailed data from German waste water treatment plants, showed that the theoretically possible production of biopolymers in Germany amounts to more than 19% of the 2016 worldwide biopolymer production. In addition, a profound estimation regarding the EU showed that in theory about 120% of the worldwide biopolymer production (in 2016) could be produced on European waste water treatment plants.
这项工作描述了在城市污水处理厂（WWTP）中将聚羟基脂肪酸酯（PHA）作为副流工艺进行生产，以及随后对德国和欧盟（EU）生产潜力的分析。因此，对来自污水处理厂的不同类型污泥的挥发性脂肪酸（VFA）生产潜力进行了测试。之后，以初沉污泥为底物，测试了一系列操作条件（温度、pH值、停留时间（RT）和出料量（WD）），以找到适合高且稳定的VFA生产的设置。第二步，进行了各种关于高PHA产量和稳定PHA组成的测试，以确定底物浓度、温度、pH值和已安装的 feast/famine 模式的循环时间的影响。半连续反应器操作实验表明，对于1913 mgVFA/(L×d)的高VFA产率（PR）和稳定的VFA组成，在pH = 6和30℃左右时，4天的短停留时间和25%的小出料量是优选的。在较低底物浓度、20℃、中性pH值和24小时循环时间下，PHA产量高达细胞干重（CDW）的28.4%。最后一步，根据德国污水处理厂的结果和详细数据进行潜力分析，结果表明德国生物聚合物的理论可能产量超过2016年全球生物聚合物产量的19%。此外，对欧盟的深入估计表明，理论上欧洲污水处理厂可以生产约为全球生物聚合物产量（2016年）120%的产品。
