Lopes Gerson Jorge Aparecido, Casella Antonio Marcelo Barbante, Oguido Ana Paula, Matsuo Tiemi
Department of Clinical Surgery, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Department of Statistics, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Arq Bras Oftalmol. 2017 Jul-Aug;80(4):252-256. doi: 10.5935/0004-2749.20170061.
: To evaluate and compare the effects of topical application and subconjunctival injection of bevacizumab on corneal neovascularization (CNV) in rabbits' eyes after chemical burning of the cornea.
: The animals were randomly distributed into four groups of five animals. In one group, the drug was instilled, while in another, it was administered by subconjunctival injection. The two procedures using bevacizumab were compared with instillation and subconjunctival injection of saline solution (S). Neovascularization was evaluated according to the size of the invasion area of new blood vessels and through computerized analysis of this area. The data were analyzed using the Kruskal-Wallis test followed by Dunn's test for two-by-two comparison of the groups, to assess the external examination of CNV. Analysis of variance was used to assess the area of CNV. P<0.05 was considered statistically significant.
: Assessing both the external examination and the invasion area of neovessels on the 5th and 10th days, there was a clear difference between the groups. The group to which saline solution had been applied showed higher scores for CNV, as well as increases in the invasion area of neovessels. Two-by-two comparison of groups revealed no significant differences. However, an analysis of the factors involved (injection vs. instillation and bevacizumab vs. saline solution) showed that injection did not differ from instillation, but that bevacizumab differed from saline solution.
: Bevacizumab showed an inhibitory effect on CNV in rabbits' eyes after chemical burning of the cornea. There was no difference between the topical or subconjunctival administration of bevacizumab in the inhibition of CNV.
评估并比较贝伐单抗局部应用和结膜下注射对兔角膜化学伤后角膜新生血管化（CNV）的影响。
将动物随机分为四组，每组五只。一组滴注药物，另一组结膜下注射药物。将使用贝伐单抗的两种给药方式与滴注和结膜下注射生理盐水（S）进行比较。根据新生血管侵袭区域的大小并通过对该区域的计算机分析来评估新生血管化情况。数据采用Kruskal-Wallis检验，随后进行Dunn检验以对各组进行两两比较，用于评估CNV的外部检查。采用方差分析评估CNV的面积。P<0.05被认为具有统计学意义。
在第5天和第10天评估外部检查和新生血管的侵袭区域时，各组之间存在明显差异。应用生理盐水的组CNV得分更高，新生血管侵袭区域也有所增加。各组两两比较未发现显著差异。然而，对相关因素（注射与滴注以及贝伐单抗与生理盐水）的分析表明，注射与滴注无差异，但贝伐单抗与生理盐水有差异。
贝伐单抗对兔角膜化学伤后的CNV有抑制作用。贝伐单抗局部或结膜下给药在抑制CNV方面无差异。
