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血清型的基因组特征分析及基于多重PCR的血清分型方案的开发

Genomic Characterization of Serotypes and Development of a Multiplex PCR-Based Serotyping Scheme.

作者信息

Rochat Tatiana, Fujiwara-Nagata Erina, Calvez Ségolène, Dalsgaard Inger, Madsen Lone, Calteau Alexandra, Lunazzi Aurélie, Nicolas Pierre, Wiklund Tom, Bernardet Jean-François, Duchaud Eric

机构信息

Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Université Paris-SaclayJouy-en-Josas, France.

Department of Fisheries, Kindai UniversityNara, Japan.

出版信息

Front Microbiol. 2017 Sep 12;8:1752. doi: 10.3389/fmicb.2017.01752. eCollection 2017.

DOI:10.3389/fmicb.2017.01752
PMID:28955320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5601056/
Abstract

is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.

摘要

是全球淡水养殖鲑鱼中一种极具破坏力的细菌病原体。到目前为止，已描述了分离株之间的血清学多样性，但潜在的分子因素仍然未知。通过对一组34株菌株进行全基因组序列分析，并结合Lorenzen和Olesen（1997）提出的血清分型方法，我们确定了血清型的关键分子决定因素。这些知识使我们能够开发出一种基于多重PCR的可靠血清分型方案，并将其应用于244株细菌分离株。结果揭示了PCR血清型与鱼类宿主物种之间的显著关联，并说明了这种方法作为一种简单且经济高效的血清群测定方法的用途。基于PCR的血清分型在一系列应用中可能是一种有用的工具，如疾病监测、选择抗细菌性冷水病的鲑鱼以及未来疫苗配方的制定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/674f894fb079/fmicb-08-01752-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/25c41a609b72/fmicb-08-01752-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/32a91f83dd2b/fmicb-08-01752-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/58c7476e5b7e/fmicb-08-01752-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/674f894fb079/fmicb-08-01752-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/25c41a609b72/fmicb-08-01752-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/32a91f83dd2b/fmicb-08-01752-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/58c7476e5b7e/fmicb-08-01752-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b0e/5601056/674f894fb079/fmicb-08-01752-g004.jpg

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