Bai Chao, Wang Peng, Fan Qiang, Fu Wei-Dong, Wang Le, Zhang Zhen-Nan, Song Zhen, Zhang Guo-Liang, Wu Jia-He
The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of SciencesBeijing, China.
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural SciencesBeijing, China.
Front Plant Sci. 2017 Sep 12;8:1579. doi: 10.3389/fpls.2017.01579. eCollection 2017.
is a notoriously invasive weed that can readily adapt to different environmental conditions. Control of this weed is difficult, and it spreads easily and causes damage to native habitats and agriculture. In this study, our goal was to investigate the molecular mechanisms that lead to the ability of to invade new habitats, to adapt to environmental stresses, and to cause damage. We developed a simple and highly effective potato virus X-based virus-induced gene silencing (VIGS) approach. The VIGS approach was first used to silence the phytoene desaturase gene, which resulted in the expected photo-bleaching phenotype. Next, the VIGS approach was used to silence two additional genes, drought-induced protein gene 15 () and salinity-induced protein gene 1 (). When was knocked down, the plants were more sensitive to drought stress than the control plants, with smaller leaves, shorter internodes, and lower biomass. The -silenced plants had lower relative water content, lower free proline levels, and higher water loss rates than the control. Silencing of significantly decreased tolerance to salinity, and the -silenced plants were withered and smaller. These results indicate that the pgR107 VIGS approach is a simple and highly effective tool for dissecting gene function in . Further experiments with the VIGS approach will enhance our understanding of the molecular mechanisms of the adaptability and plasticity of and improve our ability to combat the damage caused by this weed.
是一种臭名昭著的入侵性杂草，能够轻易适应不同的环境条件。控制这种杂草很困难，它很容易传播并对本地栖息地和农业造成破坏。在本研究中，我们的目标是探究导致其能够入侵新栖息地、适应环境胁迫并造成破坏的分子机制。我们开发了一种基于马铃薯X病毒的简单且高效的病毒诱导基因沉默（VIGS）方法。该VIGS方法首先用于沉默八氢番茄红素去饱和酶基因，这导致了预期的光漂白表型。接下来，VIGS方法被用于沉默另外两个基因，干旱诱导蛋白基因15（）和盐诱导蛋白基因1（）。当被敲低时，植株比对照植株对干旱胁迫更敏感，叶片更小节间更短且生物量更低。与对照相比，被沉默的植株相对含水量更低、游离脯氨酸水平更低且失水率更高。沉默显著降低了对盐胁迫的耐受性，且被沉默的植株枯萎且更小。这些结果表明，pgR107 VIGS方法是一种用于剖析基因功能的简单且高效的工具。使用VIGS方法的进一步实验将增进我们对其适应性和可塑性分子机制的理解，并提高我们对抗这种杂草所造成破坏的能力。
