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通过分子洞察提高作物抗旱性的策略综述。

A review on strategies for crop improvement against drought stress through molecular insights.

作者信息

Sharma Aditi, Dheer Pallavi, Rautela Indra, Thapliyal Preeti, Thapliyal Priya, Bajpai Atal Bihari, Sharma Manish Dev

机构信息

Department of Biotechnology, Graphic Era Deemed to be University, Dehradun, Uttarakhand 248001 India.

Department of Biotechnology, School of Basic and Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun, Uttarakhand 248001 India.

出版信息

3 Biotech. 2024 Jul;14(7):173. doi: 10.1007/s13205-024-04020-8. Epub 2024 Jun 4.

Abstract

The demand for food goods is rising along with the world population growth, which is directly related to the yield of agricultural crops around the world. However, a number of environmental factors, including floods, salinity, moisture, and drought, have a detrimental effect on agricultural production around the world. Among all of these stresses, drought stress (DS) poses a constant threat to agricultural crops and is a significant impediment to global agricultural productivity. Its potency and severity are expected to increase in the future years. A variety of techniques have been used to generate drought-resistant plants in order to get around this restriction. Different crop plants exhibit specific traits that contribute to drought resistance (DR), such as early flowering, drought escape (DE), and leaf traits. We are highlighting numerous methods that can be used to overcome the effects of DS in this review. Agronomic methods, transgenic methods, the use of sufficient fertilizers, and molecular methods such as clustered regularly interspaced short palindromic repeats (CRISPRs)-associated nuclease 9 (Cas9), virus-induced gene silencing (VIGS), quantitative trait loci (QTL) mapping, microRNA (miRNA) technology, and OMICS-based approaches make up the majority of these techniques. CRISPR technology has rapidly become an increasingly popular choice among researchers exploring natural tolerance to abiotic stresses although, only a few plants have been produced so far using this technique. In order to address the difficulties imposed by DS, new plants utilizing the CRISPR technology must be developed.

摘要

随着世界人口增长,对食品的需求也在增加,这与全球农作物的产量直接相关。然而,包括洪水、盐碱化、湿度和干旱在内的一些环境因素对全球农业生产产生了不利影响。在所有这些胁迫中,干旱胁迫(DS)对农作物构成持续威胁,是全球农业生产力的重大障碍。预计其强度和严重程度在未来几年还会增加。为了克服这一限制,人们采用了多种技术来培育抗旱植物。不同的作物表现出有助于抗旱(DR)的特定性状,如早花、干旱逃避(DE)和叶片性状。在本综述中,我们重点介绍了多种可用于克服干旱胁迫影响的方法。这些技术主要包括农艺方法、转基因方法、使用充足的肥料以及分子方法,如成簇规律间隔短回文重复序列(CRISPRs)相关核酸酶9(Cas9)、病毒诱导基因沉默(VIGS)、数量性状位点(QTL)定位、微小RNA(miRNA)技术和基于组学的方法。CRISPR技术已迅速成为探索对非生物胁迫自然耐受性的研究人员中越来越受欢迎的选择,尽管到目前为止仅使用该技术培育出了少数几种植物。为了应对干旱胁迫带来的困难,必须培育利用CRISPR技术的新植物。

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