利用分选酶从超短肽制备肽寡聚体。
Peptide oligomers from ultra-short peptides using sortase.
作者信息
Voloshchuk Natalya, Chen Long, Li Qiang, Liang Jun F
机构信息
Department of Chemistry, Chemical Biology, and Biomedical Engineering, Charles V. Schaefer School of Engineering and Sciences, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
出版信息
Biochem Biophys Rep. 2017 Feb 20;10:1-6. doi: 10.1016/j.bbrep.2017.02.005. eCollection 2017 Jul.
Sortase A catalyzed ligation of ultra-short peptides leads to inter/intra-molecular transpeptidation to form either linear or cyclic oligomers dependent upon the peptide length. Cyclic peptides were the main products for peptides with more than 15aa. However, for ultra-short (<15aa) peptides, cyclic oligomers became predominant in prolonged reactions. Peptides with 1-3 aminoglycines were equally active but peptide oligomers from peptide containing more than one aminoglycine were prone to hydrolysis.
分选酶A催化的超短肽连接导致分子间/分子内转肽作用，形成线性或环状寡聚物，这取决于肽的长度。对于超过15个氨基酸的肽，环状肽是主要产物。然而，对于超短（<15个氨基酸）肽，在延长反应中环状寡聚物占主导地位。含有1-3个氨基甘氨酸的肽具有同等活性，但含有多个氨基甘氨酸的肽形成的肽寡聚物易于水解。
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