Morita Makoto, Sugihara Hidemitsu, Tokunaka Kazuhiro, Tomura Arihiro, Saiga Kan, Sato Takamichi, Imamura Yasutada, Hayashi Toshihiko
Pharmaceutical Research Laboratories, Nippon Kayaku Co., Ltd., Tokyo 115-8588, Japan.
Department of Chemistry and Life Science, School of Advanced Engineering, Kogakuin University, Hachioji, Tokyo 192-0015, Japan.
Biochem Biophys Rep. 2016 Dec 8;9:128-132. doi: 10.1016/j.bbrep.2016.11.013. eCollection 2017 Mar.
This report describes the preparation and partial characterization of monoclonal antibodies that are reactive specifically with the nascently produced non-triple helical form of the type IV collagen α1 chain, designated as NTH α1(IV). These antibodies were nonreactive with the α1 chain of the type IV collagen in the triple-helical conformation. Three antibodies, #141, #179 and #370, with different epitopes in NTH α1(IV) were found to be reactive with the nascent polypeptide secreted from human normal cells and a human carcinoma cell line. The antibodies with different epitopes may provide a key method for elucidating the physiological function and tissue distribution of NTH α1(IV), which is distinct from the chain derived from triple-helical type IV collagen.
本报告描述了单克隆抗体的制备及部分特性，这些单克隆抗体能特异性地与新产生的IV型胶原α1链的非三螺旋形式发生反应，该形式被命名为NTH α1(IV)。这些抗体与处于三螺旋构象的IV型胶原α1链无反应。发现三种抗体，即#141、#179和#370，它们在NTH α1(IV)中具有不同的表位，能与人正常细胞和一种人癌细胞系分泌的新生多肽发生反应。具有不同表位的这些抗体可能为阐明NTH α1(IV)的生理功能和组织分布提供关键方法，NTH α1(IV)与源自三螺旋IV型胶原的链不同。
