Hanedan Basak, Kirbas Akin, Kandemir Fatih Mehmet, Aktas Mustafa Sinan, Yildiz Ahmet
1 Department of Internal Medicine, Faculty of Veterinary Medicine, Ataturk University , 25170 Erzurum , Turkey.
2 Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University , 25170 Erzurum , Turkey.
Acta Vet Hung. 2017 Sep;65(3):394-401. doi: 10.1556/004.2017.037.
It is known that inflammatory organ damages due to various agents, such as microorganisms including mycoplasmas, lead to oxidative stress. Nitric oxide (NO) functions as an antimicrobial agent, and arginase decreases proinflammatory cytokine release. There are very few studies on arginase activity, NO level and oxidative stress status in mycoplasmal infections. Therefore, the aim of this study was to evaluate erythrocyte arginase activity, plasma NO level and oxidative stress status in sheep with contagious agalactia. The study material consisted of 10 healthy sheep and 14 sheep with contagious agalactia characterised by mastitis, arthritis and keratoconjunctivitis. Erythrocyte arginase activity, plasma NO, malondialdehyde (MDA), total oxidant capacity (TOC) and total antioxidant capacity (TAC) levels were measured. Significant decreases in erythrocyte arginase activity and plasma TAC level (P < 0.001), and significant increases in plasma NO, MDA and TOC levels (P < 0.001) were found in the diseased sheep as compared with the healthy animals. This study suggests that contagious agalactia may cause oxidative stress due to increased plasma MDA and TOC levels and decreased plasma TAC levels, and that the decrease in erythrocyte arginase activity and increase in plasma NO level may contribute to the elimination of mycoplasmal agents causing contagious agalactia.
众所周知，由各种病原体（如包括支原体在内的微生物）引起的炎症性器官损伤会导致氧化应激。一氧化氮（NO）作为一种抗菌剂发挥作用，而精氨酸酶可减少促炎细胞因子的释放。关于支原体感染中精氨酸酶活性、NO水平和氧化应激状态的研究非常少。因此，本研究的目的是评估患有传染性无乳症的绵羊的红细胞精氨酸酶活性、血浆NO水平和氧化应激状态。研究材料包括10只健康绵羊和14只患有以乳腺炎、关节炎和角膜结膜炎为特征的传染性无乳症的绵羊。测量了红细胞精氨酸酶活性、血浆NO、丙二醛（MDA）、总氧化能力（TOC）和总抗氧化能力（TAC）水平。与健康动物相比，患病绵羊的红细胞精氨酸酶活性和血浆TAC水平显著降低（P < 0.001），血浆NO、MDA和TOC水平显著升高（P < 0.001）。本研究表明，传染性无乳症可能由于血浆MDA和TOC水平升高以及血浆TAC水平降低而导致氧化应激，并且红细胞精氨酸酶活性降低和血浆NO水平升高可能有助于消除引起传染性无乳症的支原体病原体。
