通过相分辨光学相干断层扫描术进行毛细血管红细胞测速
Capillary red blood cell velocimetry by phase-resolved optical coherence tomography.
作者信息
Tang Jianbo, Erdener Sefik Evren, Fu Buyin, Boas David A
出版信息
Opt Lett. 2017 Oct 1;42(19):3976-3979. doi: 10.1364/OL.42.003976.
Abstract
We present a phase-resolved optical coherence tomography (OCT) method to extend Doppler OCT for the accurate measurement of the red blood cell (RBC) velocity in cerebral capillaries. OCT data were acquired with an M-mode scanning strategy (repeated A-scans) to account for the single-file passage of RBCs in a capillary, which were then high-pass filtered to remove the stationary component of the signal to ensure an accurate measurement of phase shift of flowing RBCs. The angular frequency of the signal from flowing RBCs was then quantified from the dynamic component of the signal and used to calculate the axial speed of flowing RBCs in capillaries. We validated our measurement by RBC passage velocimetry using the signal magnitude of the same OCT time series data.
摘要
我们提出了一种相位分辨光学相干断层扫描（OCT）方法，以扩展多普勒OCT，用于准确测量脑毛细血管中的红细胞（RBC）速度。采用M模式扫描策略（重复A扫描）采集OCT数据，以考虑红细胞在毛细血管中的单列通过情况，然后对数据进行高通滤波，去除信号的静态成分，以确保准确测量流动红细胞的相移。然后从信号的动态成分中量化流动红细胞信号的角频率，并用于计算毛细血管中流动红细胞的轴向速度。我们使用相同OCT时间序列数据的信号幅度，通过红细胞通过测速法验证了我们的测量结果。
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