Li Yuandong, Rakymzhan Adiya, Tang Peijun, Wang Ruikang K
Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA 98195, USA.
Biomed Opt Express. 2020 May 26;11(6):3288-3300. doi: 10.1364/BOE.394649. eCollection 2020 Jun 1.
We describe a method and procedure that allows for the optical coherence tomography angiography (OCTA) and intrinsic optical signal imaging (IOSI) of cerebral blood flow and hemodynamics in fully awake mice. We detail the procedure of chronic cranial window preparation, the use of an air-lift mobile homecage to achieve stable optical recording in the head-restrained awake mouse, and the imaging methods to achieve multiparametric hemodynamic measurements. The results show that by using a collection of OCTA algorithms, the high-resolution cerebral vasculature can be reliably mapped at a fully awake state, including flow velocity measurements in penetrating arterioles and capillary bed. Lastly, we demonstrate how the awake imaging paradigm is used to study cortical hemodynamics in the mouse barrel cortex during whisker stimulation. The method presented here will facilitate optical recording in the awake, active mice and open the door to many projects that can bridge the hemodynamics in neurovascular units to naturalistic behavior.
我们描述了一种方法和程序，可用于对完全清醒小鼠的脑血流和血流动力学进行光学相干断层扫描血管造影（OCTA）和内在光学信号成像（IOSI）。我们详细介绍了慢性颅窗制备的程序、使用气举式移动家用笼在头部固定的清醒小鼠中实现稳定光学记录的方法，以及实现多参数血流动力学测量的成像方法。结果表明，通过使用一系列OCTA算法，可以在完全清醒状态下可靠地绘制高分辨率的脑血管系统，包括对穿通小动脉和毛细血管床中的血流速度进行测量。最后，我们展示了如何利用清醒成像范式研究小鼠触须刺激期间桶状皮层的皮质血流动力学。本文介绍的方法将有助于在清醒、活跃的小鼠中进行光学记录，并为许多能够将神经血管单元中的血流动力学与自然行为联系起来的项目打开大门。
