Moreau Joshua M, Cen Selena, Berger Alexandra, Furlonger Caren, Paige Christopher J
Princess Margaret Cancer Centre, University Health Network, Toronto, Canada.
Department of Immunology, University of Toronto, Toronto, Canada.
PLoS One. 2017 Sep 28;12(9):e0185509. doi: 10.1371/journal.pone.0185509. eCollection 2017.
Immature B cells are the first B cell progenitors to express a fully formed B cell receptor and are therefore subject to extensive selection processes that act to mitigate the emergence of autoreactive clones. While it is well appreciated that most B cell generation in the bone marrow is highly dependent on access to molecules present in the local milieu, the existence of extrinsically provided factors that modulate immature B cell biology is ambiguous. Nonetheless, a population of CD49b+CD90lo cells has demonstrated in vitro potential to promote immature B cell survival. Using a mouse basophil reporter strain we confirmed the identity of these CD49b+CD90lo supportive cells as basophils. However, analysis of bone marrow B cell populations following lineage specific basophil depletion demonstrates that basophils do not have a significant role in vivo in modulating immature B cell biology during steady-state conditions.
未成熟B细胞是最早表达完全形成的B细胞受体的B细胞祖细胞，因此会经历广泛的选择过程，以减少自身反应性克隆的出现。虽然人们普遍认识到骨髓中大多数B细胞的生成高度依赖于获取局部微环境中存在的分子，但调节未成熟B细胞生物学的外在提供因子的存在尚不明确。尽管如此，一群CD49b+CD90lo细胞已在体外显示出促进未成熟B细胞存活的潜力。使用小鼠嗜碱性粒细胞报告菌株，我们证实了这些CD49b+CD90lo支持细胞为嗜碱性粒细胞。然而，对谱系特异性嗜碱性粒细胞耗竭后骨髓B细胞群体的分析表明，在稳态条件下，嗜碱性粒细胞在体内调节未成熟B细胞生物学方面没有显著作用。
