Wang Yongxiang, Zhang Anyun, Yang Yongqiang, Lei Changwei, Jiang Wei, Liu Bihui, Shi Hongping, Kong Linghan, Cheng Guangyang, Zhang Xiuzhong, Yang Xin, Wang Hongning
College of Life Sciences, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR China.
College of Life Sciences, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan, PR China; "985 Project" Project Science Innovative Platform for Resource and Environment Protection of Southwestern, Sichuan University, Chengdu, Sichuan, PR China.
Int J Food Microbiol. 2017 Dec 4;262:23-30. doi: 10.1016/j.ijfoodmicro.2017.09.012. Epub 2017 Sep 23.
The aim of this study was to investigate the prevalence and characterization of Salmonella concerning the poultry industry in China. A total of 170 non-duplicate Salmonella isolates were recovered from the 1540 chicken samples. Among the Salmonella isolates from chickens, the predominant serovars were S. enterica serovar Enteritidis (S. Enteritidis) (49/170, 28.8%), S. enterica serovar Indiana (S. Indiana) (37/170, 21.8%) and S. enterica serovar California (S. California) (34/170, 20.0%). High antimicrobial resistance was observed for ciprofloxacin (68.2%), amikacin (48.2%) and cefotaxime (44.7%). Of particular concerns were the 18 S. Indiana and 17 S. California isolates, which were concurrently resistant to cefotaxime, amikacin and ciprofloxacin. The bla genes, 16S rRNA methylase genes (armA, rmtD or rmtC) and five plasmid-mediated quinolone resistance (PMQR) determinants (aac(6')-Ib-cr, oqxAB, qnrB, qepA and qnrD) were identified in 18 S. Indiana and 17 S. California isolates. To clarify their genetic correlation, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were further conducted. PFGE profiles showed that the majority of S. Indiana and S. California isolates were clonally unrelated with a standard cut-off of 85%. The results of MLST demonstrated that ST17 and ST40 were the most common ST types in S. Indiana and S. California isolates, respectively. Our findings indicated that the multiple antibiotic resistant S. Indiana and S. California isolates were widespread in chicken in China and might pose a potential threat to public health.
本研究旨在调查中国家禽业中沙门氏菌的流行情况及特征。从1540份鸡肉样本中总共分离出170株非重复的沙门氏菌菌株。在从鸡中分离出的沙门氏菌菌株中，主要血清型为肠炎沙门氏菌（肠炎沙门氏菌）（49/170，28.8%）、印第安纳沙门氏菌（印第安纳沙门氏菌）（37/170，21.8%）和加利福尼亚沙门氏菌（加利福尼亚沙门氏菌）（34/170，20.0%）。观察到对环丙沙星（68.2%）、阿米卡星（48.2%）和头孢噻肟（44.7%）的高耐药性。特别值得关注的是18株印第安纳沙门氏菌和17株加利福尼亚沙门氏菌菌株，它们同时对头孢噻肟、阿米卡星和环丙沙星耐药。在18株印第安纳沙门氏菌和17株加利福尼亚沙门氏菌菌株中鉴定出bla基因、16S rRNA甲基化酶基因（armA、rmtD或rmtC）和5种质粒介导的喹诺酮耐药性（PMQR）决定簇（aac(6')-Ib-cr、oqxAB、qnrB、qepA和qnrD）。为阐明它们的遗传相关性，进一步进行了脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）。PFGE图谱显示，大多数印第安纳沙门氏菌和加利福尼亚沙门氏菌菌株在标准截断值为85%时克隆不相关。MLST结果表明，ST17和ST40分别是印第安纳沙门氏菌和加利福尼亚沙门氏菌菌株中最常见的ST型。我们的研究结果表明，多重耐药的印第安纳沙门氏菌和加利福尼亚沙门氏菌菌株在中国鸡群中广泛存在，可能对公共卫生构成潜在威胁。
