Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for food safety Risk Assessment, Beijing, China.
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
PLoS One. 2015 Dec 9;10(12):e0144532. doi: 10.1371/journal.pone.0144532. eCollection 2015.
The prevalence of Salmonella from chicken and pig slaughterhouses in Henan, China and antimicrobial susceptibility of these isolates to antibiotics was determined. From 283 chicken samples and 240 pig samples collected, 128 and 70 Salmonella isolates were recovered with an isolation rate of 45.2 and 29.2% respectively. The predominant serovars in chicken samples were S. enterica serovar Enteritidis, S. enterica serovar Hadar and S. enterica serovar Indiana, while those in pig samples were S. enterica serovar Typhimurium, S. enterica serovar Derby and S. enterica serovar Enteritidis. Resistance to ciprofloxacin was 8.6 and 10.0% for isolates from chickens and pigs respectively, whereas resistance to cefotaxime was 5.5 and 8.6%, respectively. Multidrug resistance (resistance to three or more classes of antimicrobial agent) was markedly higher in pig isolates (57.1%) than in chicken isolates (39.8%). Of particular concern was the detection of ciprofloxacin and cefotaxime co-resistant S. enterica serovar Indiana isolates, which pose risk to public health. All 16 S. enterica serovar Indiana isolates detected were resistant to ciprofloxacin, among which 11 were co-resistant to cefotaxime. The S. enterica serovar Indiana isolates accumulated point mutations in quinolone resistance determination regions of gyrA (S83F/D87G or S83F/D87N) and parC (T57S/S80R). Two plasmid mediated quinolone resistant determinants were found with aac (6')-Ib-cr and oqxAB in 16 and 12 S. enterica serovar Indiana isolates respectively. Cefotaxime-resistance of S. enterica serovar Indiana was associated with the acquisition of a blaCTX-M-65 gene. The potential risk of ciprofloxacin and cefotaxime co-resistant S. enterica serovar Indiana infection is a significant concern due to limited alternative treatment options. Reduction of Salmonella in chicken and pig slaughterhouses, in particular, ciprofloxacin and cefotaxime co-resistant S. enterica serovar Indiana will be an important measure to reduce the public health burden of Salmonella infections.
本研究旨在调查中国河南地区鸡肉和猪肉屠宰场中沙门氏菌的流行情况及其对抗生素的药敏性。从采集的 283 份鸡肉样本和 240 份猪肉样本中，共分离出 128 株和 70 株沙门氏菌，分离率分别为 45.2%和 29.2%。在鸡肉样本中，主要血清型为肠炎沙门氏菌血清型肠炎亚种、肠炎沙门氏菌血清型哈达尔和肠炎沙门氏菌血清型印第安纳亚种，而在猪肉样本中则为鼠伤寒沙门氏菌血清型、德尔比沙门氏菌血清型和肠炎沙门氏菌血清型肠炎亚种。鸡肉源和猪肉源沙门氏菌对环丙沙星的耐药率分别为 8.6%和 10.0%，而头孢噻肟的耐药率分别为 5.5%和 8.6%。多重耐药（对 3 种或 3 种以上类别的抗菌药物耐药）在猪源沙门氏菌中显著高于鸡源沙门氏菌（57.1% vs. 39.8%）。特别值得关注的是，分离到了对环丙沙星和头孢噻肟均耐药的肠炎沙门氏菌血清型印第安纳亚种，这对公共卫生构成了威胁。检测到的 16 株肠炎沙门氏菌血清型印第安纳亚种均对环丙沙星耐药，其中 11 株对头孢噻肟也耐药。这些肠炎沙门氏菌血清型印第安纳亚种在喹诺酮类药物耐药决定区 gyra（S83F/D87G 或 S83F/D87N）和 parC（T57S/S80R）发生点突变。在 16 株和 12 株肠炎沙门氏菌血清型印第安纳亚种中分别发现了两种质粒介导的喹诺酮耐药决定子 aac（6'）-Ib-cr 和 oqxAB。头孢噻肟耐药与 blaCTX-M-65 基因的获得有关。由于可供选择的治疗方法有限，对环丙沙星和头孢噻肟均耐药的肠炎沙门氏菌血清型印第安纳亚种感染的潜在风险令人担忧。减少鸡肉和猪肉屠宰场中的沙门氏菌，特别是减少环丙沙星和头孢噻肟耐药的肠炎沙门氏菌血清型印第安纳亚种，将是降低沙门氏菌感染公共卫生负担的重要措施。
