Nakagomi T, Kassell N F, Sasaki T, Lehman R M, Torner J C, Hongo K, Lee J H
Department of Neurological Surgery, University of Virginia School of Medicine, Charlottesville.
J Neurosurg. 1988 May;68(5):757-66. doi: 10.3171/jns.1988.68.5.0757.
The effect of endothelium removal on the contractile responses to KCl, hemoglobin, serotonin (5-HT), norepinephrine (NE), prostaglandin (PG)F2 alpha, PGD2, and PGE2 was investigated in canine and rabbit basilar arteries by an isometric tension-recording method. In canine basilar arteries, endothelium removal elevated the dose-response curves to 5-HT, PGF2 alpha, and PGD2, and PGE2, but not to KCl, hemoglobin, or NE. In rabbit basilar arteries, on the other hand, removal of the endothelium elevated the dose-response curves to 5-HT, NE, PGF2 alpha, and PGD2, but not to KCl or hemoglobin. Neither contractile nor inhibitory response was elicited by PGE2 in rabbit basilar arteries. Contraction induced by 5-HT and NE following endothelium removal had a much more pronounced effect in rabbit basilar arteries than in canine basilar arteries. These results suggest that, following endothelium removal, abolition of the spontaneous release of endothelium-derived relaxing factor is the most probable mechanism of the enhanced vasocontraction. Since endothelial damage results from subarachnoid hemorrhage, the aforementioned mechanism of vasocontraction enhancement may play a role in the pathogenesis of cerebral vasospasm.
采用等长张力记录法，研究了犬和兔基底动脉中去除内皮对氯化钾、血红蛋白、血清素（5-羟色胺）、去甲肾上腺素、前列腺素F2α、前列腺素D2和前列腺素E2收缩反应的影响。在犬基底动脉中，去除内皮使对5-羟色胺、前列腺素F2α、前列腺素D2和前列腺素E2的剂量反应曲线升高，但对氯化钾、血红蛋白或去甲肾上腺素则无此作用。另一方面，在兔基底动脉中，去除内皮使对5-羟色胺、去甲肾上腺素、前列腺素F2α和前列腺素D2的剂量反应曲线升高，但对氯化钾或血红蛋白则无此作用。前列腺素E2在兔基底动脉中既不引起收缩反应也不引起抑制反应。去除内皮后5-羟色胺和去甲肾上腺素诱导的收缩在兔基底动脉中比在犬基底动脉中具有更明显的作用。这些结果表明，去除内皮后，内皮源性舒张因子自发释放的消除是血管收缩增强最可能的机制。由于蛛网膜下腔出血会导致内皮损伤，上述血管收缩增强机制可能在脑血管痉挛的发病机制中起作用。
