Cha Ji-Yun, Jeon Yong-Deok, Xin Mingjie, Kim Do-Kuk, Lee Hoon-Yeon, Kim Bo-Ram, Hwang Sung-Woo, Kim Dae-Ki, Jin Jong-Sik, Lee Young-Mi
a Department of Oriental Pharmacy, College of Pharmacy , Wonkwang Oriental Medicine Research Institute, Wonkwang University , Iksan , Jeollabuk-do , Republic of Korea.
b Department of Oriental Medicine Resources , Chonbuk National University , Iksan , Jeollabuk-do , Republic of Korea.
Biosci Biotechnol Biochem. 2017 Nov;81(11):2178-2185. doi: 10.1080/09168451.2017.1373590. Epub 2017 Sep 29.
The aim of this study is to examine the anti-inflammatory effect of Euphorbia supina (ES) ethanol extract in dextran sulfate sodium (DSS)-induced experimental colitis model. ES was per orally administered at different doses of 4 or 20 mg/kg body weight with 5% DSS in drinking water for 7 days. Twenty mg/kg of ES administration regulated body weight decrease, recovered colon length shortening, and increased disease activity index score and myeloperoxidase level in DSS-induced colitis. Histological features showed that 20 mg/kg of ES administration suppressed edema, mucosal damage, and the loss of crypts induced by DSS. Furthermore, ES suppressed the expressions of COX-2, iNOS, NF-kB, IkBα, pIkBα in colon tissue. These findings demonstrated a possible effect of amelioration of ulcerative colitis and could be clinically applied.
本研究旨在探讨地锦草乙醇提取物（ES）在葡聚糖硫酸钠（DSS）诱导的实验性结肠炎模型中的抗炎作用。将ES以4或20mg/kg体重的不同剂量经口给药，同时在饮用水中加入5%的DSS，持续7天。给予20mg/kg的ES可调节体重减轻，恢复结肠长度缩短，并增加DSS诱导的结肠炎中的疾病活动指数评分和髓过氧化物酶水平。组织学特征显示，给予20mg/kg的ES可抑制DSS诱导的水肿、黏膜损伤和隐窝丢失。此外，ES抑制结肠组织中COX-2、iNOS、NF-κB、IkBα、pIkBα的表达。这些发现证明了ES对溃疡性结肠炎可能具有改善作用，且可应用于临床。
