Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.
College of Food Science and Technology, Guangdong Ocean University, Haida Road 1, Mazhang, Zhanjiang 524088, Guangdong, China.
Food Chem. 2018 Feb 15;241:104-112. doi: 10.1016/j.foodchem.2017.08.080. Epub 2017 Aug 24.
Pigment-binding proteins play important roles in crustacean shell colour change. In this study, a red colour-related pigment-binding protein, designated LvPBP75, was purified from the shell of Litopenaeus vannamei. HPLC and PAGE analysis showed that LvPBP75 was a homogeneous monomer with molecular mass of 75kDa. Peptide mass fingerprint analysis revealed that LvPBP75 belonged to hemocyanin, and the released pigment from heated LvPBP75 showed a λ at 481nm in acetone. The significant red-colour change temperatures were detected at 30 and 80°C, respectively. Based on the determined amino acid fragments, a full-length cDNA of LvPBP75 was cloned and sequenced. The ORF encodes a protein of 662 amino acids having 80% identity with penaeidae hemocyanin. These results strongly suggest a novel function of hemocyanin, namely binding with pigment, and its involvement in L. vannamei shell colour change.
色素结合蛋白在甲壳动物壳色变化中发挥重要作用。本研究从凡纳滨对虾（Litopenaeus vannamei）壳中纯化得到一种与红色相关的色素结合蛋白，命名为 LvPBP75。HPLC 和 PAGE 分析表明，LvPBP75 是一种均一的单体，分子量为 75kDa。肽质量指纹图谱分析表明，LvPBP75 属于血蓝蛋白，从加热的 LvPBP75 释放的色素在丙酮中显示 λ 在 481nm。分别检测到显著的红色变色温度为 30 和 80°C。基于测定的氨基酸片段，克隆并测序了 LvPBP75 的全长 cDNA。ORF 编码一种 662 个氨基酸的蛋白质，与对虾血蓝蛋白具有 80%的同源性。这些结果强烈表明血蓝蛋白具有结合色素的新功能，并参与了凡纳滨对虾壳色变化。
