Department of Odontology/cariology, Umeå University, SE-901 87 Umeå, Sweden.
Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden.
EBioMedicine. 2017 Oct;24:205-215. doi: 10.1016/j.ebiom.2017.09.027. Epub 2017 Sep 21.
Dental caries, which affects billions of people, is a chronic infectious disease that involves Streptococcus mutans, which is nevertheless a poor predictor of individual caries development. We therefore investigated if adhesin types of S.mutans with sucrose-independent adhesion to host DMBT1 (i.e. SpaP A, B or C) and collagen (i.e. Cnm, Cbm) match and predict individual differences in caries development. The adhesin types were measured in whole saliva by qPCR in 452 12-year-old Swedish children and related to caries at baseline and prospectively at a 5-year follow-up. Strains isolated from the children were explored for genetic and phenotypic properties. The presence of SpaP B and Cnm subtypes coincided with increased 5-year caries increment, and their binding to DMBT1 and saliva correlated with individual caries scores. The SpaP B subtypes are enriched in amino acid substitutions that coincided with caries and binding and specify biotypes of S. mutans with increased acid tolerance. The findings reveal adhesin subtypes of S. mutans that match and predict individual differences in caries development and provide a rationale for individualized oral care.
龋齿影响着全球数十亿人，是一种慢性传染病，涉及变形链球菌，但它并不能很好地预测个体龋齿的发展。因此，我们研究了变形链球菌是否具有与宿主 DMBT1（即 SpaP A、B 或 C）和胶原蛋白（即 Cnm、Cbm）蔗糖非依赖性黏附的黏附素类型，并预测龋齿发展的个体差异。在 452 名 12 岁的瑞典儿童的全唾液中通过 qPCR 测量了这些黏附素类型，并在基线和 5 年随访时前瞻性地与龋齿相关。还探索了从儿童中分离出的菌株的遗传和表型特性。SpaP B 和 Cnm 亚型的存在与 5 年龋齿增量增加有关，它们与 DMBT1 和唾液的结合与个体龋齿评分相关。SpaP B 亚型富含与龋齿和结合相关的氨基酸取代，指定了具有更高耐酸能力的变形链球菌生物型。研究结果揭示了与龋齿发展的个体差异相匹配并可预测个体差异的变形链球菌黏附素亚型，并为个体化口腔护理提供了依据。
