来自不同龋病状况泰国儿童的变形链球菌的多位点序列分型和表型特性。
Multilocus sequence typing and phenotypic properties of Streptococcus mutans from Thai children with different caries statuses.
机构信息
Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Rajthevi, Bangkok, 10400, Thailand.
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan.
出版信息
BMC Oral Health. 2024 Sep 11;24(1):1063. doi: 10.1186/s12903-024-04759-9.
BACKGROUND
Streptococcus mutans is studied for its acidogenic and aciduric characteristics, notably its biofilm formation in the presence of sucrose, toward its role in the caries process. Variations in both genotype and phenotype have been reported among clinical isolates of S. mutans. This study aimed to examine genotypic and phenotypic characteristics of S. mutans obtained from Thai children with varying caries statuses.
METHODS
We determined the presence of S. mutans and caries status in 395 children aged 3-4 years. From 325 children carrying S. mutans, we selected 90 with different caries statuses-caries-free (CF; n = 30), low severity of caries (LC; n = 30), or high severity of caries (HC; n = 30). Three isolates of S. mutans were taken from each child, thus, a total of 270 isolates were obtained. Multilocus sequence typing (MLST) was used to genotype the isolates and assess their clonal relationships. The properties, including biofilm formation, collagen binding, and acid production and tolerance were also evaluated.
RESULTS
Children with carious lesions showed a higher detection rate and number of S. mutans in saliva than those without caries. S. mutans from individuals with HC status showed the lowest biofilm formation ability, while this group had the highest detection rate of collagen-binding isolates. There was no difference in acid production or tolerance by caries status. Genotyping by MLST did not reveal any clone of S. mutans specific to CF status. This result remained even when we included MLST data from the open-access PubMLST database. MLST did identify clones containing only strains from caries-affected hosts, but tests of their phenotypic properties did not reveal any differences between S. mutans from these clones and clones that were from both caries-free and caries-affected children.
CONCLUSIONS
The clonal relationships of S. mutans indicated by MLST were not associated with the status of dental caries in the host.
背景
变形链球菌因其产酸和耐酸特性而受到研究，尤其是其在存在蔗糖的情况下形成生物膜，这与其在龋齿过程中的作用有关。临床分离株的基因型和表型均有报道。本研究旨在检查来自泰国不同龋病状态儿童的变形链球菌的基因型和表型特征。
方法
我们确定了 395 名 3-4 岁儿童的变形链球菌存在情况和龋病状况。在携带变形链球菌的 325 名儿童中，我们选择了 90 名具有不同龋病状况的儿童，包括无龋（CF；n=30）、低龋严重程度（LC；n=30）或高龋严重程度（HC；n=30）。从每个孩子中取出 3 株变形链球菌，因此共获得 270 株分离株。多位点序列分型（MLST）用于对分离株进行基因分型并评估其克隆关系。还评估了包括生物膜形成、胶原结合以及产酸和耐酸能力在内的特性。
结果
有龋病病变的儿童唾液中变形链球菌的检出率和数量均高于无龋病的儿童。HC 状态个体的变形链球菌生物膜形成能力最低，而该组的胶原结合分离株检出率最高。产酸或耐酸能力与龋病状况无关。MLST 基因分型未发现 CF 状态特定的变形链球菌克隆。当我们包括来自开放访问 PubMLST 数据库的 MLST 数据时，结果仍然如此。MLST 确实确定了仅包含受龋齿影响宿主来源菌株的克隆，但对其表型特性的测试并未发现这些克隆与来自无龋和受龋齿影响儿童的克隆之间的任何差异。
结论
MLST 指示的变形链球菌的克隆关系与宿主的龋齿状况无关。
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