Jain Priyanka, Sharma Vinay, Dubey Himanshu, Singh Pankaj Kumar, Kapoor Ritu, Kumari Mandeeep, Singh Jyoti, Pawar Deepak V, Bisht Deepak, Solanke Amolkumar U, Mondal T K, Sharma T R
ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi-110012, India.
Department of Bioscience & Biotechnology, Banasthali University, Tonk, Rajasthan-304022, India.
Bioinformation. 2017 Aug 31;13(8):249-255. doi: 10.6026/97320630013249. eCollection 2017.
Rice blast disease caused by a fungus Magnaporthae oryzae is one of the most important biotic factors that severely damage the rice crop. Several molecular approaches are now being applied to tackle this issue in rice. It is of interest to study long non-coding RNA (lncRNA) in rice to control the disease. lncRNA, a non-coding transcript that does not encode protein, is known to play an important role in gene regulation of various biological processes. Here we describe a computational pipeline to identify lncRNA from a resistant rice line. The number of lncRNA found in resistant line was 1429, 1927 and 1981 in mock and M. oryzae (ZB13 and Zhong) inoculated samples, respectively. Functional classification of these lncRNA reveals a higher number of long intergenic non-coding RNA compared to antisense lncRNA in both mock and M. oryzae inoculated resistant rice lines. Many intergenic lncRNA candidates were identified from resistant rice line and their role to regulate the resistance mechanism in rice during M. oryzae invasion is implied.
由稻瘟病菌引起的稻瘟病是严重损害水稻作物的最重要生物因素之一。目前正在应用多种分子方法来解决水稻中的这一问题。研究水稻中的长链非编码RNA（lncRNA）以控制该病具有重要意义。lncRNA是一种不编码蛋白质的非编码转录本，已知在各种生物过程的基因调控中发挥重要作用。在此，我们描述了一种从抗性水稻品系中鉴定lncRNA的计算流程。在模拟接种和接种稻瘟病菌（ZB13和中）的样品中，抗性品系中发现的lncRNA数量分别为1429、1927和1981。这些lncRNA的功能分类显示，在模拟接种和接种稻瘟病菌的抗性水稻品系中，与反义lncRNA相比，长链基因间非编码RNA的数量更多。从抗性水稻品系中鉴定出许多基因间lncRNA候选物，这暗示了它们在稻瘟病菌侵染期间调节水稻抗性机制中的作用。
