Andreassen Monica, Hjertholm Hege, Cravedi Jean-Pierre, Grob Koni, Alexander Jan, Nygaard Unni C
Norwegian Institute of Public Health, PO Box 4404, NO-0403 Oslo, Norway.
Toxalim, INRA, ENVT, INP-EIPurpan, Université de Toulouse, F-31027 Toulouse, France.
Toxicol Rep. 2017 Feb 16;4:104-112. doi: 10.1016/j.toxrep.2017.02.002. eCollection 2017.
Pristane and other adjuvants based on mineral oil saturated hydrocarbons (MOSH) may induce autoimmunity in rodents after intradermal injection; however there is a lack of information on immune effects after oral MOSH exposure. The aim of our study was to determine the impact of dietary exposure to pristane and other MOSH on the development of autoimmune arthritis. Dark Agouti (DA) rats were given feed containing 4000 mg/kg pristane or a broad MOSH mixture in various concentrations (0-4000 mg/kg) for 90 days, or a single intradermal injection of 200 μl pristane (positive control). Arthritis scores, and serum and splenocyte markers previously associated with arthritis development, were determined. All rats injected with pristane displayed arthritis symptoms and higher levels of certain serum markers. None of the rats fed pristane or MOSH developed arthritis symptoms or demonstrated clear changes in any measured arthritis-associated biological markers in serum or splenocytes. The absence of clinical arthritis symptoms or any increase in common arthritis-associated biological markers in sera and spleen following dietary exposure to pristane or a broad MOSH mixture in a sub-chronic rat model of arthritis suggest that dietary MOSH have low capacity to promote development of autoimmunity.
pristane和其他基于矿物油饱和烃（MOSH）的佐剂在皮内注射后可能会在啮齿动物中诱发自身免疫；然而，关于口服MOSH暴露后的免疫效应的信息却很少。我们研究的目的是确定饮食中接触pristane和其他MOSH对自身免疫性关节炎发展的影响。将深色刺豚鼠（DA）大鼠喂食含有4000mg/kg pristane或不同浓度（0-4000mg/kg）的广泛MOSH混合物的饲料90天，或皮内单次注射200μl pristane（阳性对照）。测定关节炎评分以及先前与关节炎发展相关的血清和脾细胞标志物。所有注射pristane的大鼠均出现关节炎症状和某些血清标志物水平升高。喂食pristane或MOSH的大鼠均未出现关节炎症状，也未在血清或脾细胞中任何测量的与关节炎相关的生物学标志物上表现出明显变化。在亚慢性关节炎大鼠模型中，饮食中接触pristane或广泛的MOSH混合物后，血清和脾脏中没有临床关节炎症状或任何常见的与关节炎相关的生物学标志物增加，这表明饮食中的MOSH促进自身免疫发展的能力较低。
