细胞健康状况对哺乳动物细胞中蛋白质折叠状态的影响。
Impact of cellular health conditions on the protein folding state in mammalian cells.
作者信息
Inomata Kohsuke, Kamoshida Hajime, Ikari Masaomi, Ito Yutaka, Kigawa Takanori
机构信息
RIKEN Quantitative Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
出版信息
Chem Commun (Camb). 2017 Oct 10;53(81):11245-11248. doi: 10.1039/c7cc06004a.
By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.
通过进行细胞内核磁共振实验，我们已经证明细胞中的蛋白质折叠状态受到细胞健康状况的显著影响。在压力培养条件下，hAK1在细胞中会发生变性，而在持续供应新鲜培养基的细胞中，它仍保持功能并正确折叠。
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