Cody Robert B, Tamura Jun, Downard Kevin M
JEOL USA Inc., Peabody, MA, USA.
JEOL Ltd., Akishima, Tokyo, Japan.
J Mass Spectrom. 2018 Jan;53(1):58-64. doi: 10.1002/jms.4033.
The ability to rapidly identify and quantitate, over a wide range of concentrations, anthocyanins in food and therapeutic products is important to ensuring their presence at medicinally significant levels. Sensitive, yet mild, analysis conditions are required given their susceptibility to degradation and transformation. Paper spray ionization has been used to detect and quantify the levels of anthocyanin levels in extracts of fresh and dried elderberries, and elderberry stems, as well as 3 commercially available nutraceutical formulations. The component cyanidin glucosides, including cyanidin-3-sambubioside, cyanidin-3-glucoside, cyanidin-3,5-diglucoside, cyanidin-3-sambubioside-5-glucoside, and the aglycone cyanidin, were readily detected in a range of sources. Quantitation was achieved by establishing a calibration plot from dilutions of a stock solution of cyanidin-3,5-diglucoside containing malvidin-3,5-diglucoside as an internal standard at a fixed concentration. The same standard was used to quantify the anthocyanin content in the fruit and nutraceutical formulations. Wide 5-fold variations in anthocyanin concentration were detected in the nutraceutical formulations from different suppliers ranging from 1050 to 5430 mg/100 g. These concentrations compared with 500 to 2370 mg/100 g measured in the dried stems and fruit, respectively.
在很宽的浓度范围内快速鉴定和定量食品及治疗产品中的花青素，对于确保其在具有医学意义的水平上存在非常重要。鉴于花青素易降解和转化，需要灵敏但温和的分析条件。纸喷雾电离已用于检测和定量新鲜和干燥接骨木果、接骨木茎提取物以及3种市售营养制剂中的花青素水平。在一系列来源中很容易检测到包括矢车菊素-3-接骨木二糖苷、矢车菊素-3-葡萄糖苷、矢车菊素-3,5-二葡萄糖苷、矢车菊素-3-接骨木二糖苷-5-葡萄糖苷和苷元矢车菊素在内的矢车菊素葡萄糖苷成分。通过以含有固定浓度的锦葵色素-3,5-二葡萄糖苷作为内标的矢车菊素-3,5-二葡萄糖苷储备溶液的稀释液建立校准曲线来实现定量。使用相同的标准物来定量水果和营养制剂中的花青素含量。在不同供应商的营养制剂中检测到花青素浓度有5倍的广泛差异，范围从1050至5430毫克/100克。这些浓度分别与在干燥茎和果实中测得的500至2370毫克/100克相比。
