miR-622 表达下调通过靶向调控 c-Myc 促进胆管癌细胞的增殖、迁移和侵袭。

Decrease of miR-622 expression promoted the proliferation, migration and invasion of cholangiocarcinoma cells by targeting regulation of c-Myc.

机构信息

Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, 410005, China.

Department of Hepatobiliary Surgery, Hainan Provincial People's Hospital, Haikou, 570311, China.

出版信息

Biomed Pharmacother. 2017 Dec;96:7-13. doi: 10.1016/j.biopha.2017.09.043. Epub 2017 Nov 24.

DOI:10.1016/j.biopha.2017.09.043

PMID:28961507

Abstract

OBJECTIVE

To explore the mechanism of miR-622 in regulating the proliferation, migration and invasion of cholangiocarcinoma (CCA) cells.

MATERIALS AND METHODS

Quantitative real-time PCR was conducted to measure the expression of miR-622 and c-Myc in CCA tissues and cell lines. Protein level of c-Myc was measured by Western blot. The effect of miR-622 on cell proliferation, migration and invasion was analyzed by MTT assay and Transwell chamber migration assay. Luciferase reporter assay was performed to measure the effect of miR-622 on c-Myc.

RESULTS

miR-622 expression was downregulated in both CCA tissues and cell lines, while c-Myc expression was uregulated. Overexpression of miR-622 in CCA cells was statistically correlated with a decrease of cell proliferation, migration and invasion, while inhibition of miR-622 made an inverse result. We also proved c-Myc was identified as a target gene of miR-622 in CCA. Moreover, we found overexpression of c-Myc can strengthen the effects of miR-622 on the proliferation, migration and invasion of CCA cells.

CONCLUSION

Decrease of miR-622 promotes the proliferation, migration and invasion of CCA cells by directly targeting c-Myc.

摘要

目的

探讨 miR-622 调控胆管癌细胞增殖、迁移和侵袭的机制。

材料与方法

采用实时定量 PCR 检测 miR-622 和 c-Myc 在胆管癌组织和细胞系中的表达。Western blot 检测 c-Myc 蛋白水平。MTT 法和 Transwell 迁移实验分析 miR-622 对细胞增殖、迁移和侵袭的影响。荧光素酶报告基因实验检测 miR-622 对 c-Myc 的作用。

结果

miR-622 在胆管癌组织和细胞系中表达下调，而 c-Myc 表达上调。CCA 细胞中 miR-622 的过表达与细胞增殖、迁移和侵袭的减少呈统计学相关，而 miR-622 的抑制则产生相反的结果。我们还证明 c-Myc 是 CCA 中 miR-622 的靶基因。此外，我们发现过表达 c-Myc 可以增强 miR-622 对 CCA 细胞增殖、迁移和侵袭的影响。