亮氨酸脑啡肽在猫回盲括约肌直接和间接作用的证据。

Evidence for a direct and indirect action of leucine enkephalin at the feline ileocecal sphincter.

作者信息

Bertiger G, Ouyang A, Reynolds J C, Cohen S

机构信息

Dept. of Medicine, University of Pennsylvania, Philadelphia 19104.

出版信息

Life Sci. 1988;42(18):1697-703. doi: 10.1016/0024-3205(88)90035-5.

DOI:10.1016/0024-3205(88)90035-5

PMID:2896285

Abstract

An in vitro whole organ bath preparation was used to examine the effects of leucine enkephalin on the cat ileocecal sphincter (ICS) intraluminal pressure and myoelectric activity. The bath allowed separation of the bathing media surrounding the ICS and the ileum. Leucine enkephalin (2 x 10(-7) M) when added to the ileal bathing medium caused a delayed increase in ICS spike activity and pressure which was blocked by tetrodotoxin (10(-5)M). In contrast, leucine enkephalin (2 x 10(-7)M) added directly to the ICS bathing medium caused an immediate spike-associated contractile response which was tetrodotoxin-resistant. Thus both an indirect and direct opiate action at the ICS was demonstrated.

摘要

采用体外全器官浴槽制备法研究亮氨酸脑啡肽对猫回盲括约肌（ICS）腔内压力和肌电活动的影响。该浴槽可将围绕ICS和回肠的浴液分开。当向回肠浴液中加入亮氨酸脑啡肽（2×10⁻⁷ M）时，ICS锋电位活动和压力出现延迟性升高，这一现象被河豚毒素（10⁻⁵ M）阻断。相反，直接向ICS浴液中加入亮氨酸脑啡肽（2×10⁻⁷ M）会引起即刻的锋电位相关收缩反应，该反应对河豚毒素具有抗性。因此，证明了在ICS处存在间接和直接的阿片类作用。