Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Concepción, Chile.
Department of Marine Biotechnology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, BC, Mexico.
Fish Shellfish Immunol. 2017 Dec;71:69-75. doi: 10.1016/j.fsi.2017.09.061. Epub 2017 Sep 28.
The population of the Chilean endemic marine gastropod Concholepas concholepas locally called "loco" has dramatically decreased in the past 50 years as a result of intense activity of local fisheries and high environmental variability observed along the Chilean coast, including episodes of hypoxia, changes in sea surface temperature, ocean acidification and diseases. In this study, we set out to explore the molecular basis of C. concholepas to cope with biotic stressors such as exposure to the pathogenic bacterium Vibrio anguillarum. Here, 454pyrosequencing was conducted and 61 transcripts related to the immune response in this muricid species were identified. Among these, the expression of six genes (CcNFκβ, CcIκβ, CcLITAF, CcTLR, CcCas8 and CcCath) involved in the regulation of inflammatory, apoptotic and immune processes upon stimuli, were evaluated during the first 33 h post challenge (hpc). The results showed that CcTLR, CcCas8 and CcCath have an initial response at 4 hpc, evidencing an up-regulation from 4 to 24 hpc. Notably, the response of CcNFKB occurred 2 h later with a statistically significant up-regulation at 6 hpc and 10 hpc. Furthermore, the challenge with V. anguillarum induced a statistically significant down-regulation of CcIKB between 2 and 10 hpc as well as a down-regulation of CcLITAF between 2 and 4 hpc followed in both cases by an up-regulation between 24 and 33 hpc. This work describes the first transcriptomic effort to characterize the immune response of C. concholepas and constitutes a valuable transcriptomic resource for future efforts to develop sustainable aquaculture and conservations tools for this endemic marine snail species.
智利特有海洋腹足纲动物 Concholepas concholepas （当地称为“loco”）的数量在过去 50 年中急剧减少，这是由于当地渔业的高强度活动以及智利沿海地区观察到的高环境变异性的结果，包括缺氧事件、海面温度变化、海洋酸化和疾病。在这项研究中，我们着手探索 C. concholepas 应对生物胁迫的分子基础，例如暴露于致病性细菌 Vibrio anguillarum。在这里，我们进行了 454 焦磷酸测序，鉴定了该物种 61 种与免疫反应相关的转录本。在这些转录本中，评估了 6 种基因（CcNFκβ、CcIκβ、CcLITAF、CcTLR、CcCas8 和 CcCath）的表达，这些基因涉及在受到刺激后炎症、凋亡和免疫过程的调节，在挑战后 33 小时内（hpc）进行了表达。结果表明，CcTLR、CcCas8 和 CcCath 在 4 hpc 时具有初始反应，在 4 至 24 hpc 时有上调。值得注意的是，CcNFKB 的反应晚了 2 小时，在 6 hpc 和 10 hpc 时有统计学意义的上调。此外，V. anguillarum 的挑战导致 CcIKB 在 2 至 10 hpc 之间的统计学显著下调，CcLITAF 在 2 至 4 hpc 之间的下调，随后在这两种情况下，在 24 至 33 hpc 之间的上调。这项工作描述了 C. concholepas 免疫反应的首次转录组学研究，并为未来开发这种特有海洋蜗牛可持续水产养殖和保护工具提供了有价值的转录组资源。
