巴西萨尔瓦多 ST-103 克隆复合体 C 群脑膜炎奈瑟菌的传播。

Dissemination of the ST-103 clonal complex serogroup C meningococci in Salvador, Brazil.

机构信息

Gonçalo Moniz Research Institute, Oswaldo Cruz Foundation, Brazil Ministry of Health, Rua Waldemar Falcão, 141, Candeal, Salvador 40296-710, Brazil; School of Pharmacy, Federal University of Bahia, Rua Barão do Jeremoabo, n° 147, Ondina, Salvador, 40170-115, Brazil.

Gonçalo Moniz Research Institute, Oswaldo Cruz Foundation, Brazil Ministry of Health, Rua Waldemar Falcão, 141, Candeal, Salvador 40296-710, Brazil; Municipal Secretary of Health of Salvador, Rua da Grécia, 3, Comercio, Salvador 40010-010, Brazil.

出版信息

Microbes Infect. 2018 Jan;20(1):19-24. doi: 10.1016/j.micinf.2017.09.010. Epub 2017 Sep 27.

DOI:10.1016/j.micinf.2017.09.010

PMID:28962886

Abstract

Invasive meningococcal disease (IMD) is a major public health problem worldwide. An epidemic of serogroup C (NmC) IMD occurred in 2010 in the city of Salvador. In this study, we describe the antigenic and genetic characterization of meningococcal isolates collected from meningitis cases in Salvador from 2001 to 2012. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed for the analysis of IMD isolates. A total of 733 cases were identified, and the serogroup was determined for 391 (53.0%) of these. Most cases were caused by NmC (53%) or B (47%). The most prevalent strains were B:4,7:P1.19,15 (32.9%; 129/391) and C:23:P1.14-6 (28.6%; 112/391). Based on PFGE/MLST analysis, 71.3% (77/108 PFGE-tested isolates) clustered as two clones of sequence type ST-3779 and ST-3780, both belonging to the ST-103 clonal complex. ST-3779 has been detected in Salvador since 1996 and together with ST-3780 became predominant after 2005. There was a predominance of C:23:P1.14-6, ST-3779/3780 in Salvador during the period of 2007-2012, establishing a major clonal lineage, which remained in the community for a long time; this has serious implications for public health, particularly in terms of prevention and control strategies of IMD.

摘要

侵袭性脑膜炎球菌病（IMD）是全球范围内的一个主要公共卫生问题。2010 年，萨尔瓦多市爆发了 C 群（NmC）脑膜炎球菌病疫情。在本研究中，我们描述了 2001 年至 2012 年期间从萨尔瓦多脑膜炎病例中收集的脑膜炎奈瑟菌分离株的抗原和遗传特征。进行了脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）分析 IMD 分离株。共确定了 733 例病例，其中 391 例（53.0%）确定了血清型。大多数病例由 NmC（53%）或 B（47%）引起。最常见的菌株是 B:4,7:P1.19,15（32.9%；129/391）和 C:23:P1.14-6（28.6%；112/391）。基于 PFGE/MLST 分析，71.3%（108 个 PFGE 测试分离株中的 77 个）聚类为两个序列型 ST-3779 和 ST-3780 的克隆，均属于 ST-103 克隆复合体。ST-3779 自 1996 年以来在萨尔瓦多被检测到，与 ST-3780 一起，在 2005 年后成为主要优势型。2007 年至 2012 年期间，萨尔瓦多以 C:23:P1.14-6、ST-3779/3780 为主，建立了一个主要的克隆谱系，在社区中存在了很长时间；这对公共卫生，特别是 IMD 的预防和控制策略，产生了严重影响。