Brannan Robert G, Peters Trisha E, Black Kathleen J, Kukor Benjamin J
School of Applied Health Sciences and Wellness, Ohio University, Athens, OH, USA.
J Sci Food Agric. 2018 Apr;98(6):2210-2214. doi: 10.1002/jsfa.8706. Epub 2017 Nov 15.
The objective of this study was to characterize the ability of extracts from nine varieties of pawpaw pulp standardized to the phenolics level of 0.1% grape seed extract (GSE) on inhibition of the formation of thiobarbituric reactive substances (TBARS) in a turkey model system. The antioxidant activity of the extracts was also determined using four common assays.
Over the 240 min sampling time, the standardized pawpaw extracts from all nine varieties were as effective as GSE in inhibiting TBARS formation in turkey muscle homogenate compared to the untreated control. Extracts from all pawpaw varieties and GSE began to inhibit TBARS formation at 60 min of incubation, and by 240 min TBARS were reduced from 35 μmol malondialdehyde kg tissue in the homogenate to which no antioxidant was added to 4-18 μmol malondialdehyde kg tissue in the antioxidant-enriched extracts. There does not appear to be a clear relationship between inhibition of TBARS and any of the antioxidant capacity measurements (ORAC, DPPH inhibition, reducing potential as measured by FRAP assay, or pyrogallol red bleaching).
The results of this research indicate that there is potential to add value to pawpaw as a functional food source of natural antioxidants, particularly in meat products. © 2017 Society of Chemical Industry.
本研究的目的是在火鸡模型系统中,表征九种番木瓜果肉提取物在标准化至葡萄籽提取物(GSE)酚类水平为0.1%时对硫代巴比妥酸反应性物质(TBARS)形成的抑制能力。还使用四种常见测定方法确定了提取物的抗氧化活性。
在240分钟的采样时间内,与未处理的对照相比,所有九个品种的标准化番木瓜提取物在抑制火鸡肌肉匀浆中TBARS形成方面与GSE一样有效。所有番木瓜品种和GSE的提取物在孵育60分钟时开始抑制TBARS形成,到240分钟时,TBARS从未添加抗氧化剂的匀浆中每千克组织35微摩尔丙二醛减少到富含抗氧化剂的提取物中每千克组织4 - 18微摩尔丙二醛。TBARS的抑制与任何抗氧化能力测量值(氧自由基吸收能力、DPPH抑制、通过铁离子还原抗氧化能力测定法测量的还原电位或邻苯三酚红褪色)之间似乎没有明显关系。
本研究结果表明,番木瓜作为天然抗氧化剂的功能性食品来源具有增值潜力,特别是在肉类产品中。© 2017化学工业协会。