Laboratoire de Biotechnologie de l'Olivier, Centre de Biotechnologie de Borj Cedria, BP 901, 2050 Hammam-Lif, Tunisia.
Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain; Functional Food Research and Development Centre (CIDAF), Health-Science Technological Park, Avda. Del Conocimiento s/n, 18100 Granada, Spain.
Food Res Int. 2017 Oct;100(Pt 3):454-461. doi: 10.1016/j.foodres.2016.12.002. Epub 2016 Dec 10.
The aim of this study was to investigate the changes in the phenolic profile in different organs (buds, flowers, fruits and leaves) from Chemlali olive variety cultivated in the Center of Tunisia according to development stage. Phenolics recovery was carried out using aqueous methanol from freeze-dried powdered tissues. The extracts were then analyzed by using high performance liquid chromatography coupled to time of flight and ion trap mass spectrometry detectors. Qualitatively, secoiridoids, flavonoids, simple phenols, cinnamic acid derivatives and lignans were identified in the analyzed extracts. Quantitatively, floral buds showed the highest phenolic contents compared to the other tissues under study. The highest content of secoiridoids was observed for unripe fruit extract, whereas the highest content of flavonoids was registered for floral buds.
本研究旨在根据发育阶段,研究突尼斯中部 Chemlali 橄榄品种不同器官(芽、花、果实和叶)中酚类物质组成的变化。采用从冷冻干燥的粉末组织中提取的甲醇水溶液进行酚类物质的回收。然后使用高效液相色谱与飞行时间和离子阱质谱联用检测器对提取物进行分析。定性分析结果表明,在所分析的提取物中鉴定出了木脂素、类黄酮、简单酚类、肉桂酸衍生物和木质素。定量分析结果表明,与其他研究组织相比,花蕾中的酚类物质含量最高。未成熟果实提取物中的次级胆汁酸含量最高,而花蕾中的类黄酮含量最高。