Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP 1173, Sfax 3038, Tunisia.
Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland.
Molecules. 2023 Jun 17;28(12):4829. doi: 10.3390/molecules28124829.
The large quantity of olive roots resulting from a large number of old and unfruitful trees encouraged us to look for ways of adding value to these roots. For this reason, the current research work is devoted to the valorization of olive roots by identifying active phytochemicals and assessing their biological activities, including the cytotoxicity and antiviral potential of different extracts from the Chemlali cultivar. The extract, obtained by ultrasonic extraction, was analyzed using the liquid chromatography-mass spectrometry technique (LC-MS). The cytotoxicity was evaluated through the use of the microculture tetrazolium assay (MTT) against VERO cells. Subsequently, the antiviral activity was determined for HHV-1 (Human Herpesvirus type 1) and CVB3 (Coxsackievirus B3) replication in the infected VERO cells. LC-MS analysis allowed the identification of 40 compounds, classified as secoiridoids (53%), organic acids (13%), iridoids (10%), lignans (8%), caffeoylphenylethanoid (5%), phenylethanoids (5%),sugars and derivatives (2%), phenolic acids (2%), and flavonoids (2%). It was found that extracts were not toxic to the VERO cells. Moreover, the extracts did not influence the appearance of HHV-1 or CVB3 cytopathic effects in the infected VERO cells and failed to decrease the viral infectious titer.
大量的老而无果的橄榄树产生了大量的橄榄根，这促使我们寻找增加这些橄榄根附加值的方法。出于这个原因，目前的研究工作致力于通过鉴定活性植物化学物质并评估其生物活性来实现橄榄根的增值，包括 Chemlali 品种的不同提取物的细胞毒性和抗病毒潜力。通过超声提取获得的提取物使用液相色谱-质谱联用技术（LC-MS）进行分析。通过使用微培养四唑测定法（MTT）对 VERO 细胞进行评估，评估了细胞毒性。随后，在感染的 VERO 细胞中测定了 HHV-1（人疱疹病毒 1 型）和 CVB3（柯萨奇病毒 B3）复制的抗病毒活性。LC-MS 分析鉴定出 40 种化合物，分为裂环烯醚萜类（53%）、有机酸类（13%）、环烯醚萜类（10%）、木脂素类（8%）、咖啡酰苯乙醇类（5%）、苯乙醇类（5%）、糖和衍生物（2%）、酚酸类（2%）和类黄酮类（2%）。结果发现，提取物对 VERO 细胞没有毒性。此外，提取物不会影响感染的 VERO 细胞中 HHV-1 或 CVB3 的细胞病变效应，也不能降低病毒感染滴度。
