Graduate Institute of Environmental Engineering, National Central University, No. 300, Zhongda Rd., Zhongli District, Taoyuan City 32001, Taiwan.
Institute of Microbiology and Immunology, National Yang Ming University, Taipei, Taiwan.
Mar Pollut Bull. 2017 Dec 15;125(1-2):416-425. doi: 10.1016/j.marpolbul.2017.09.042. Epub 2017 Sep 28.
4-t-Octylphenol (4-t-OP) has become a serious environmental concern due to the endocrine disruption in animals and humans. The biodegradation of 4-t-OP by pure cultures has been extensively investigated only in bacteria and wood-decaying fungi. In this study we isolated and identified 14 filamentous fungal strains from wastewater samples in Taiwan using 4-t-OP as a sole carbon and energy source. The isolates were identified based on sequences from different DNA regions. Of 14 fungal isolates, 10 strains grew effectively on solid medium with a wide variety of endocrine disrupting chemicals as the sole carbon and energy source. As revealed by high-performance liquid chromatography analysis, the most effective 4-t-OP degradation (>70%) in liquid medium was observed in Fusarium falciforme after 15days. To our knowledge, this is the first report on the degradation of 4-t-OP as a sole carbon and energy source by non-ligninolytic fungi.
4-叔辛基苯酚（4-t-OP）由于对动物和人类的内分泌干扰而成为严重的环境问题。仅在细菌和木材腐朽真菌中广泛研究了纯培养物对 4-t-OP 的生物降解。在这项研究中，我们从台湾的废水样本中分离并鉴定了 14 株丝状真菌菌株，这些菌株以 4-t-OP 作为唯一的碳源和能源。根据来自不同 DNA 区域的序列对分离物进行了鉴定。在 14 株真菌分离物中，有 10 株在固体培养基上有效生长，其作为唯一的碳源和能源的种类繁多。高效液相色谱分析表明，在 15 天后，镰刀菌（Fusarium falciforme）对 4-t-OP 的降解效果最佳（>70%）。据我们所知，这是首次报道非木质素分解真菌以 4-t-OP 作为唯一碳源和能源进行降解的研究。
